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Publicação:
Impact of sewage sludge on the soil bacterial communities by DNA microarray analysis

dc.contributor.authorVal-Moraes, Silvana P. [UNESP]
dc.contributor.authorMarcondes, Jackson [UNESP]
dc.contributor.authorCarareto Alves, Lucia M. [UNESP]
dc.contributor.authorLemos, Eliana G. M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:32:44Z
dc.date.available2014-05-20T15:32:44Z
dc.date.issued2011-09-01
dc.description.abstractSewage sludge has been used as organic manure to replace chemical fertilizer. The aim of this study was to evaluate the effect of doses of sewage sludge on the soil bacterial community by DNA microarray analysis. A microarray phylochip containing 1,560 partial sequences of 16S rRNA from the most common strains of bacteria was developed for bioprospection. Soil plots from an experimental field in Brazil were assessed with or without sludge treatment containing different doses of nitrogen based on that recommended for maize cultivation. The microarray technique was useful for quickly assessing changes in the bacterial communities and a high variation was observed, mainly in soil treated with high doses of sludge. While sludge containing 25 kg N/ha favored an in crease in the number of members in various phyla, on the other hand sludge with the higher dose regarding to 200 kg N/ha caused a reduction in the number of members in almost all phyla. Proteobacteria often dominant in soils was specifically affected. This study highlights the spread of bacteria to new environments and provides direct information about bacterial composition at specific habitats. Our results have shown that bacterial community structure was greatly affected by sludge application.en
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Agr & Vet, BR-14884900 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Agr & Vet, BR-14884900 São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent1997-2003
dc.identifierhttp://dx.doi.org/10.1007/s11274-011-0660-3
dc.identifier.citationWorld Journal of Microbiology & Biotechnology. New York: Springer, v. 27, n. 9, p. 1997-2003, 2011.
dc.identifier.doi10.1007/s11274-011-0660-3
dc.identifier.issn0959-3993
dc.identifier.urihttp://hdl.handle.net/11449/41563
dc.identifier.wosWOS:000293793900005
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofWorld Journal of Microbiology & Biotechnology
dc.relation.ispartofjcr2.100
dc.relation.ispartofsjr0,604
dc.rights.accessRightsAcesso restritopt
dc.sourceWeb of Science
dc.subjectBacterial community structureen
dc.subject16S rRNAen
dc.subjectOrganic manureen
dc.subjectNitrogen impacten
dc.titleImpact of sewage sludge on the soil bacterial communities by DNA microarray analysisen
dc.typeArtigopt
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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