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&ITShort communication&IT:The first report of &ITCyberlindnera rhodanensis&IT associated with clinical bovine mastitis

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Elsevier B.V.

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Article

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Abstract

An acute case of clinical mastitis in a Holstein cow from second lactation is reported here. A milk sample from the affected quarter was cultured on 5% bovine blood agar and incubated at 37 degrees C for 72 h. After 24 h of incubation, numerous colonies of yeast were observed: the Candida characteristic was not detected by CHROMagar Candida (Difco, Franklin Lakes, NJ). The DNA extraction of the isolate was performed, and DNA. was subjected to amplification and sequencing of the D1/D2 region of the large subunit rRNA gene. The sequences were aligned using Mega 7.0 and used for searching GenBank by BLASTn (Basic Local Alignment Search Tool for nucleotides), revealing 98% of identity with Cyberlindnera rhodanensis. To date, this is the first report of this yeast associated with clinical bovine mastitis.

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Keywords

yeast, dairy science, milk, intramammary infection

Language

English

Citation

Journal Of Dairy Science. New York: Elsevier Science Inc, v. 101, n. 1, p. 581-583, 2018.

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Item type:Unit,
Faculdade de Medicina Veterinária e Zootecnia
FMVZ
Campus: Botucatu


Item type:Unit,
Instituto de Biociências
IBB
Campus: Botucatu


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