Characterization and in vitro cytocompatibility of an acid-etched titanium surface
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FapUNIFESP (SciELO)
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Acesso aberto
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Resumo
The aims of this study were to characterize the microstructure of a commercially pure titanium (cpTi) surface etched with HCl/H 2SO 4 (AE-cpTi) and to investigate its in vitro cytocompatibility compared to turned cpTi (T-cpTi). T-cpTi showed a grooved surface and AE-cpTi revealed a surface characterized by the presence of micropits. Surface parameters indicated that the AE-cpTi surface is more isotropic and present a greater area compared to T-cpTi. The oxide film thickness was similar between both surfaces; however, AE-cpTi presented more Ti and O and less C. Osteoblastic cell proliferation, alkaline phosphatase activity, and bone-like nodule formation were greater on T-cpTi than on AE-cpTi. These results show that acid etching treatment produced a surface with different topographical and chemical features compared to the turned one, and such surface modification affected negatively the in vitro cytocompatibility of cpTi as demonstrated by decreasing culture growth and expression of osteoblastic phenotype.
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Palavras-chave
Cell culture Cytocompatibility Osteoblast Surface treatment Titanium alkaline phosphatase biological marker biomaterial biomedical and dental materials carbon hydrochloric acid sulfuric acid titanium titanium dioxide bone development cell adhesion cell culture cell division cell proliferation cell survival chemistry comparative study cytology dental acid etching drug effect human interferometry jaw materials testing osteoblast phenotype scanning electron microscopy surface property X ray photoelectron spectroscopy Acid Etching, Dental Alkaline Phosphatase Alveolar Process Biocompatible Materials Biological Markers Carbon Cell Adhesion Cell Division Cell Proliferation Cell Survival Cells, Cultured Dental Materials Humans Hydrochloric Acid Interferometry Materials Testing Microscopy, Electron, Scanning Osteoblasts Osteogenesis Phenotype Photoelectron Spectroscopy Sulfuric Acids Surface Properties
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Inglês
Citação
Brazilian Dental Journal, v. 21, n. 1, p. 3-11, 2010.
