Publication: The effects of low laser irradiation on angiogenesis in injured rat tibiae
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Undergraduate course
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Abstract
The influence of He-Ne laser radiation on the formation of new blood vessels in the bone marrow compartment of a regenerating area of the mid-cortical diaphysis of the tibiae of young adult rats was studied. A small hole was surgically made with a dentistry burr in the tibia and the injured area received a daily laser therapy over 7 or 14 days transcutaneously starting 24 h from surgery. Incident energy density dosages of 31.5 and 94.5 Jcm-2 were applied during the period of the tibia wound healing investigated. Light microscopic examination of histological sections of the injured area and quantification of the newly-formed blood vessels were undertaken. Low-level energy treatment accelerated the deposition of bone matrix and histological characteristics compatible with an active recovery of the injured tissue. He-Ne laser therapy significantly increased the number of blood vessels after 7 days irradiation at an energy density of 94.5 Jcm-2, but significantly decreased the number of vessels in the 14-day irradiated tibiae, independent of the dosage. These effects were attributed to laser treatment, since no significant increase in blood vessel number was detected between 8 and 15 non-irradiated control tibiae. Molecular mechanisms involved in low-level laser therapy of angiogenesis in post-traumatic bone regeneration needs further investigation.
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Keywords
Angiogenesis, Bone wound healing, He-Ne laser, Rat, Tibia, angiogenesis, animal experiment, animal model, animal tissue, bone injury, bone marrow, bone matrix, bone regeneration, comparative study, controlled study, cortical bone, density, diaphysis, helium neon laser, histology, low level laser therapy, male, microscopy, molecular dynamics, nonhuman, quantitative analysis, radiation dose, rat, tibia, wound healing, Animals, Helium, Laser Therapy, Low-Level, Lasers, Male, Neon, Neovascularization, Physiologic, Rats, Rats, Wistar, Time Factors, Wound Healing, Animalia
Language
English
Citation
Histology and Histopathology, v. 19, n. 1, p. 43-48, 2004.
