Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
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Graduate program
Undergraduate course
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Publisher
Sociedade Brasileira de Microbiologia
FapUNIFESP (SciELO)
FapUNIFESP (SciELO)
Type
Article
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Acesso aberto
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Abstract
We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.
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Language
English
Citation
Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 42, n. 2, p. 774-777, 2011.
