Publicação: Stimulation of MMP-1 and CCL2 by NAMPT in PDL cells
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2013-09-23
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Periodontitis is an inflammatory disease caused by pathogenic microorganisms and characterized by the destruction of the periodontium. Obese individuals have an increased risk of periodontitis, and elevated circulating levels of adipokines, such as nicotinamide phosphoribosyltransferase (NAMPT), may be a pathomechanistic link between both diseases. The aim of this in vitro study was to examine the regulation of periodontal ligament (PDL) cells by NAMPT and its production under inflammatory and infectious conditions. NAMPT caused a significant upregulation of 9 genes and downregulation of 3 genes, as analyzed by microarray analysis. Eight of these genes could be confirmed by real-time PCR: NAMPT induced a significant upregulation of EGR1, MMP-1, SYT7, ITPKA, CCL2, NTM, IGF2BP3, and NRP1. NAMPT also increased significantly the MMP-1 and CCL2 protein synthesis. NAMPT was significantly induced by interleukin-1β and the periodontal microorganism P. gingivalis. NAMPT may contribute to periodontitis through upregulation of MMP-1 and CCL2 in PDL cells. Increased NAMPT levels, as found in obesity, may therefore represent a mechanism whereby obesity could confer an increased risk of periodontitis. Furthermore, microbial and inflammatory signals may enhance the NAMPT synthesis in PDL cells and thereby contribute to the increased gingival and serum levels of this adipokine, as found in periodontitis. © 2013 Marjan Nokhbehsaim et al.
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early growth response factor 1, inositol trisphosphate 3 kinase, insulin like growth factor 2 mRNA binding protein 3, interleukin 1beta, interstitial collagenase, monocyte chemotactic protein 1, neuropilin 1, neurotrimin, nicotinamide phosphoribosyltransferase, peptides and proteins, potassium channel tetramerization domain containing protein 12, RGS4 protein, synaptotagmin, synaptotagmin 7, transmembrane 4 l six family member 20, unclassified drug, cell type, controlled study, cytokine production, disease association, down regulation, human, human cell, in vitro study, microarray analysis, obesity, periodontal ligament, periodontal ligament cell, periodontitis, Porphyromonas gingivalis, priority journal, protein synthesis, upregulation
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Inglês
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Mediators of Inflammation, v. 2013.
