Short communication: Greater intensity of estrous expression is associated with improved embryo viability from superovulated Holstein heifers

Abstract

The aim of this study was to determine the association between estrous expression, measured using a breeding indicator and an automated activity monitor (AAM), and the success of embryo collection after superovulation. Holstein heifers (n = 51; 10.5 to 14.5 mo, and 325.0 +/- 21.1 kg of body weight) were superovulated (n = 69 events) for the collection of embryos using a protocol based on sequential administration of FSH for follicle superstimulation and GnRH to induce ovulation. Artificial insemination (AI) was performed twice, once at the moment of GnRH administration and again 12 h later, using thawed, sexed semen. Ovaries were scanned via ultrasonography on the day of the first AI to count the total number of preovulatory follicles and 7 d later for the total number of corpora lutea present. Embryos were collected 7 d post-AI, counted, and assessed for viability. A breeding indicator (Estrotect, Rockway Inc., Spring Valley, WI) and a collar-mounted AAM (CowScout Activity Monitoring System, GEA, Dusseldorf, Germany) were used to measure standing mounts and an algorithmic estimate of estrous expression, respectively. A score for the breeding indicator was given as follows: score 1 = 100% of the indicator was intact; score 2 = 50% of the indicator was rubbed off; score 3 = greater than 50% of the indicator was rubbed off. Estrous expression detected by the AAM was quantified through the relative increase in physical activity and duration of time spent above a set threshold. Data were analyzed by ANOVA using the MIXED procedures of SAS (SAS Institute Inc., Cary, NC). The number of follicles present at AI was not affected by estrous expression. The mean (+/- SD) ovulatory response was 67.5 +/- 26.3%. We found an effect of estrous expression as detected by the breeding indicator on the ovulatory response (42.1 +/- 8.0, vs. 78.2 +/- 9.0, vs. 74.0 +/- 4.9%, for scores 1, 2, and 3, respectively) but not from the AAM. Heifers that had a score of 3 (versus those with scores of 1 and 2) on the breeding indicator had a greater number of embryos (4.1 +/- 0.5, vs. 1.2 +/- 1.0, vs. 1.8 +/- 1.0 embryos), and a greater percentage of these embryos were viable (43.1 +/- 0.05, vs. 35.5 +/- 0.1, vs. 34.3 +/- 0.1%). Similarly, heifers that showed a greater intensity of activity (as measured by the AAM) had a greater number of embryos collected (10.2 +/- 1.2 vs. 6.0 +/- 1.3 embryos), and a greater percentage of those embryos were viable (53.1 +/- 5.0 vs. 23.4 +/- 5.1%). Longer-duration estrus episodes were associated with a higher percentage of viable embryos (51.2 +/- 5.2 vs. 25.3 +/- 5.3%). In conclusion, stronger estrous intensity was associated with a greater number of total embryos collected and a greater percentage of viable embryos. These results suggest that monitoring the intensity of estrus could be used to predict superovulatory response as well as embryo quality in Holstein heifers.