Publicação: In vitro PGF2 alpha production by endometrium and corpus luteum explants from pregnant and nonpregnant diestrus bitches and placental explants from pregnant bitches
dc.contributor.author | Luz, Marcelo Rezende | |
dc.contributor.author | Bertan, Claudia Maria | |
dc.contributor.author | Binelli, Mario | |
dc.contributor.author | Lopes, Maria Denise [UNESP] | |
dc.contributor.institution | Universidade Federal do Espírito Santo (UFES) | |
dc.contributor.institution | Universidade de São Paulo (USP) | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2014-02-26T17:26:40Z | |
dc.date.accessioned | 2014-05-20T13:41:39Z | |
dc.date.available | 2014-02-26T17:26:40Z | |
dc.date.available | 2014-05-20T13:41:39Z | |
dc.date.issued | 2006-10-01 | |
dc.description.abstract | To better understand the process of slow luteal regression of the nonpregnant cycle in dogs and the acute luteolysis that occurs prepartum, the present study investigated in vitro PGF2 alpha production by the endometrium, corpus luteum and placental explants obtained at known times of the cycle from pregnant bitches (days 63, 64 and immediately postpartum; day 0 = estimated day of the ovulatory LH surge) and from nonpregnant diestrus bitches (approximately days 65, 75 and 85). Both basal PGF2 alpha production and its production in the presence of the protein kinase C (PKC) stimulator 12,13-phorbol dibutyrate (PDBu) were determined. For PDBu-supplemented incubations, mean PGF2 alpha production (pg/mL/mg/6 h) by endometrium explants of the nonpregnant bitches in late diestrus was highest on day 65 (205 +/- 87) and reduced to low levels (38 +/- 17 and 11 +/- 11) on days 75 and 85, respectively. The production by corpus luteum explants from these bitches was significantly less on day 65 (46 +/- 14) than that of the day 65 endometrium explants, and was slightly increased on day 85 (103 +/- 52). The corresponding mean PGF2 alpha production by the endometrium explants of pregnant bitches was on average much greater (i.e., two to three-fold) compared to nonpregnant bitches (P < 0.01) and involved high concentrations at day 64 (1523 +/- 467) and postpartum, compared to somewhat lower levels on day 63 (830 +/- 65); luteal PGF production (165 +/- 4) was also higher than in nonpregnant bitches around day 65. For pregnant bitches, PGF production per gram of tissue in the endometrium explants was greater than for the CL or placenta explants (180 +/- 37). Therefore, the endometrium of the pregnant bitch has an increased capability to produce PGF2a immediately prepartum, which on a tissue weight basis, exceeds that of either corpora lutea or the placenta. However, assuming a larger mass of placental tissue in vivo, we inferred that the placenta may contribute substantially to peripheral PGF concentrations. (c) 2006 Published by Elsevier B.V. | en |
dc.description.affiliation | Fed Univ Espirito Santo, Dept Anim Sci & Rural Econ, CCA, BR-29500000 Alegre, ES, Brazil | |
dc.description.affiliation | Univ São Paulo, Dept Anim Reprod, CBRA, LFEM, BR-05508 São Paulo, Brazil | |
dc.description.affiliation | São Paulo State Univ, Dept Anim Reprod & Vet Radiol, Sch Vet Med & Anim Sci FMVZ, BR-18618000 Botucatu, SP, Brazil | |
dc.description.affiliationUnesp | São Paulo State Univ, Dept Anim Reprod & Vet Radiol, Sch Vet Med & Anim Sci FMVZ, BR-18618000 Botucatu, SP, Brazil | |
dc.format.extent | 1442-1447 | |
dc.identifier | http://dx.doi.org/10.1016/j.theriogenology.2006.01.037 | |
dc.identifier.citation | Theriogenology. New York: Elsevier B.V., v. 66, n. 6-7, p. 1442-1447, 2006. | |
dc.identifier.doi | 10.1016/j.theriogenology.2006.01.037 | |
dc.identifier.issn | 0093-691X | |
dc.identifier.lattes | 6666129914663018 | |
dc.identifier.lattes | 5720162493397851 | |
dc.identifier.orcid | 0000-0003-4211-0649 | |
dc.identifier.uri | http://hdl.handle.net/11449/14433 | |
dc.identifier.wos | WOS:000240887300007 | |
dc.language.iso | eng | |
dc.publisher | Elsevier B.V. | |
dc.relation.ispartof | Theriogenology | |
dc.relation.ispartofjcr | 2.136 | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | bitch | pt |
dc.subject | luteal regression | pt |
dc.subject | PGF2 alpha | pt |
dc.subject | PDBu | pt |
dc.subject | parturition | pt |
dc.title | In vitro PGF2 alpha production by endometrium and corpus luteum explants from pregnant and nonpregnant diestrus bitches and placental explants from pregnant bitches | en |
dc.type | Artigo | |
dcterms.license | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
dcterms.rightsHolder | Elsevier B.V. | |
dspace.entity.type | Publication | |
unesp.author.lattes | 6666129914663018 | |
unesp.author.lattes | 5720162493397851[2] | |
unesp.author.orcid | 0000-0002-5995-3944[3] | |
unesp.author.orcid | 0000-0003-4211-0649[2] | |
unesp.campus | Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária e Zootecnia, Botucatu | pt |
unesp.department | Reprodução Animal e Radiologia Veterinária - FMVZ | pt |
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