Publicação:
Age related obesity-induced shortening of GLUT4 mRNA poly(A) tail length in rat gastrocnemius skeletal muscle

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dc.contributor.authorSeraphim, Patricia Monteiro
dc.contributor.authorNunes, Maria Tereza
dc.contributor.authorGiannocco, Gisele
dc.contributor.authorMachado, Ubiratan Fabres
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:22:36Z
dc.date.available2014-05-27T11:22:36Z
dc.date.issued2007-09-30
dc.description.abstractObese insulin resistant animals and humans have shown reduced GLUT4 gene expression. Yet, in skeletal muscle, discrepancy between mRNA and protein regulation has been frequently observed, suggesting a post-transcriptional modulation. We investigated the GLUT4 expression in adipose tissue and muscle of obese 12-month-old (12-mo) rats, comparing with lean 2-month-old (2-mo) animals. Obesity was accompanied by insulin resistance, and 65% reduction (P < 0.01) in GLUT4 mRNA and protein in adipose tissue. However, in muscle, despite increased (P < 0.05) mRNA content, GLUT4 protein was unchanged. RNase H and poly(A) test assays showed a reduction (P < 0.01) of ∼80 adenines in the GLUT4 mRNA poly(A) tail of muscle from 12-mo rats, recognizing that the poly(A) tail length correlates with translation efficiency. Concluding, age related obesity of 12-mo rats involves suppression of GLUT4 expression in adipose tissue; however, in muscle, GLUT4 mRNA content increases, but with a shorter poly(A) tail, thus unchanging the protein content. © 2007 Elsevier B.V. All rights reserved.en
dc.description.affiliationDepartment of Physiotherapy University of State of Sao Paulo, Presidente Prudente, Sao Paulo
dc.description.affiliationDepartment of Physiology and Biophysics Institute of Biomedical Sciences University of Sao Paulo, Av. Prof. Lineu Prestes 1524, 05505-900 Sao Paulo, SP
dc.format.extent80-87
dc.identifierhttp://dx.doi.org/10.1016/j.mce.2007.07.004
dc.identifier.citationMolecular and Cellular Endocrinology, v. 276, n. 1-2, p. 80-87, 2007.
dc.identifier.doi10.1016/j.mce.2007.07.004
dc.identifier.issn0303-7207
dc.identifier.lattes0411008599070871
dc.identifier.orcid0000-0003-2145-6640
dc.identifier.scopus2-s2.0-34548240639
dc.identifier.urihttp://hdl.handle.net/11449/69896
dc.language.isoeng
dc.relation.ispartofMolecular and Cellular Endocrinology
dc.relation.ispartofjcr3.563
dc.relation.ispartofsjr1,629
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectGLUT4
dc.subjectInsulin resistance
dc.subjectObesity
dc.subjectPAT assay
dc.subjectPoly(A) tail
dc.subjectRNase H assay
dc.subjectglucose transporter 4
dc.subjectinsulin
dc.subjectmessenger RNA
dc.subjectpolyadenylic acid
dc.subjectribonuclease
dc.subjectadipose tissue
dc.subjectaging
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectcontrolled study
dc.subjectgastrocnemius muscle
dc.subjectinsulin resistance
dc.subjectnonhuman
dc.subjectobesity
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectrat
dc.subjectskeletal muscle
dc.subjecttail
dc.subjectAdenine
dc.subjectAdipose Tissue, White
dc.subjectAging
dc.subjectAnimals
dc.subjectGene Expression Regulation
dc.subjectGlucose Transporter Type 4
dc.subjectInsulin Resistance
dc.subjectMale
dc.subjectMuscle, Skeletal
dc.subjectPoly A
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectRNA, Messenger
dc.titleAge related obesity-induced shortening of GLUT4 mRNA poly(A) tail length in rat gastrocnemius skeletal muscleen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dspace.entity.typePublication
unesp.author.lattes0411008599070871[1]
unesp.author.orcid0000-0003-2145-6640[1]

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