Publicação: Delimiting the Origin of a B Chromosome by FISH Mapping, Chromosome Painting and DNA Sequence Analysis in Astyanax paranae (Teleostei, Characiformes)
dc.contributor.author | Silva, Duilio M. Z. de A. [UNESP] | |
dc.contributor.author | Pansonato-Alves, Jose Carlos [UNESP] | |
dc.contributor.author | Utsunomia, Ricardo [UNESP] | |
dc.contributor.author | Araya-Jaime, Cristian [UNESP] | |
dc.contributor.author | Ruiz-Ruano, Francisco J. | |
dc.contributor.author | Daniel, Sandro Natal [UNESP] | |
dc.contributor.author | Hashimoto, Diogo Teruo [UNESP] | |
dc.contributor.author | Oliveira, Claudio [UNESP] | |
dc.contributor.author | Camacho, Juan Pedro M. | |
dc.contributor.author | Porto-Foresti, Fabio [UNESP] | |
dc.contributor.author | Foresti, Fausto [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Univ Granada | |
dc.date.accessioned | 2014-12-03T13:09:13Z | |
dc.date.available | 2014-12-03T13:09:13Z | |
dc.date.issued | 2014-04-15 | |
dc.description.abstract | Supernumerary (B) chromosomes have been shown to contain a wide variety of repetitive sequences. For this reason, fluorescent in situ hybridisation (FISH) is a useful tool for ascertaining the origin of these genomic elements, especially when combined with painting from microdissected B chromosomes. In order to investigate the origin of B chromosomes in the fish species Astyanax paranae, these two approaches were used along with PCR amplification of specific DNA sequences obtained from the B chromosomes and its comparison with those residing in the A chromosomes. Remarkably, chromosome painting with the one-arm metacentric B chromosome probe showed hybridization signals on entire B chromosome, while FISH mapping revealed the presence of H1 histone and 18S rDNA genes symmetrically placed in both arms of the B chromosome. These results support the hypothesis that the B chromosome of A. paranae is an isochromosome. Additionally, the chromosome pairs Nos. 2 or 23 are considered the possible B chromosome ancestors since both contain syntenic H1 and 18S rRNA sequences. The analysis of DNA sequence fragments of the histone and rRNA genes obtained from the microdissected B chromosomes showed high similarity with those obtained from 0B individuals, which supports the intraspecific origin of B chromosomes in A. paranae. Finally, the population hereby analysed showed a female-biased B chromosome presence suggesting that B chromosomes in this species could influence sex determinism. | en |
dc.description.affiliation | Univ Estadual Paulista, Dept Morfol, Inst Biociencias, Sao Paulo, Brazil | |
dc.description.affiliation | Univ Estadual Paulista, CAUNESP, Sao Paulo, Brazil | |
dc.description.affiliation | Univ Granada, Dept Genet, Granada, Spain | |
dc.description.affiliation | Univ Estadual Paulista, Dept Ciencias Biol, Fac Ciencias, Sao Paulo, Brazil | |
dc.description.affiliationUnesp | Univ Estadual Paulista, Dept Morfol, Inst Biociencias, Sao Paulo, Brazil | |
dc.description.affiliationUnesp | Univ Estadual Paulista, CAUNESP, Sao Paulo, Brazil | |
dc.description.affiliationUnesp | Univ Estadual Paulista, Dept Ciencias Biol, Fac Ciencias, Sao Paulo, Brazil | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.description.sponsorshipId | FAPESP: 11/16825-3 | |
dc.description.sponsorshipId | FAPESP: 10/17009-2 | |
dc.format.extent | 10 | |
dc.identifier | http://dx.doi.org/10.1371/journal.pone.0094896 | |
dc.identifier.citation | Plos One. San Francisco: Public Library Science, v. 9, n. 4, 10 p., 2014. | |
dc.identifier.doi | 10.1371/journal.pone.0094896 | |
dc.identifier.file | WOS000336922600105.pdf | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.lattes | 3815338174165832 | |
dc.identifier.uri | http://hdl.handle.net/11449/112082 | |
dc.identifier.wos | WOS:000336922600105 | |
dc.language.iso | eng | |
dc.publisher | Public Library Science | |
dc.relation.ispartof | PLOS ONE | |
dc.relation.ispartofjcr | 2.766 | |
dc.relation.ispartofsjr | 1,164 | |
dc.rights.accessRights | Acesso aberto | |
dc.source | Web of Science | |
dc.title | Delimiting the Origin of a B Chromosome by FISH Mapping, Chromosome Painting and DNA Sequence Analysis in Astyanax paranae (Teleostei, Characiformes) | en |
dc.type | Artigo | |
dcterms.rightsHolder | Public Library Science | |
dspace.entity.type | Publication | |
unesp.author.lattes | 0804793944846367 | |
unesp.author.lattes | 0804793944846367 | |
unesp.author.orcid | 0000-0002-2507-9814[9] | |
unesp.author.orcid | 0000-0002-5391-301X[5] | |
unesp.author.orcid | 0000-0002-0235-6831[6] | |
unesp.author.orcid | 0000-0003-3580-7384[2] | |
unesp.campus | Universidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatu | pt |
unesp.campus | Universidade Estadual Paulista (UNESP), Faculdade de Ciências, Bauru | pt |
unesp.department | Ciências Biológicas - FC | pt |
unesp.department | Morfologia - IBB | pt |
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