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First genetic identification of Cryptosporidium parvum subtype IIaA14G2R1in beef cattle in Brazil

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dc.contributor.authorHeckler, Rafael Pereira
dc.contributor.authorBorges, Dyego Gonçalves Lino
dc.contributor.authorBacha, Flávia Barbieri
dc.contributor.authorOnizuka, Marcel Kenzo Vilalba
dc.contributor.authorTeruya, Letícia E. Silva
dc.contributor.authorNeves, Juliana Paniago Lordelo
dc.contributor.authorLeal, Cássia Rejane Brito
dc.contributor.authorLemos, Ricardo Antônio Amaral de
dc.contributor.authorMeireles, Marcelo Vasconcelos [UNESP]
dc.contributor.authorBorges, Fernando de Almeida
dc.contributor.institutionUniversidade Federal de Mato Grosso do Sul (UFMS)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-12-07T15:38:53Z
dc.date.available2015-12-07T15:38:53Z
dc.date.issued2015-10-01
dc.description.abstractThe presence of Cryptosporidium spp. in a cattle herd registered with an outbreak of diarrhea was investigated and the the molecular subtyping of Cryptosporidium parvum was characterized. Fecal samples from 85 Nellore beef cattle (Bos indicus) were collected and examined with Ziehl-Neelsen modified staining method. Fifty-four cattle (63.52%) had Cryptosporidium spp. oocysts in their feces. Fragments of genes encoding the 18S ribosomal RNA subunit and a 60-kDa glycoprotein (gp60) were amplified by nested PCR accomplished in the 11 most heavily parasitized samples, and the amplicons were sequenced. Eight of the 11 analyzed samples were positive for 18S rRNA sequences and identified monospecific infections with C. parvum. Seven samples were positive for gp60 and identified subtypes IIaA15G2R1 (6/11) and IIaA14G2R1 (1/11). This report is the first for C. parvum subtype IIaA14G2R1 in beef cattle in Brazil.en
dc.description.affiliationUniversidade Federal de Mato Grosso do Sul, UFMS, Campo Grande, MS 79070-900, Brazil
dc.description.affiliationFaculdade de Medicina Veterinária-Universidade Estadual Paulista-UNESP, Araçatuba, Brazil
dc.description.affiliationUniversidade Federal de Mato Grosso do Sul, UFMS, Campo Grande, MS 79070-900, Brazil
dc.description.affiliationUnespFaculdade de Medicina Veterinária-Universidade Estadual Paulista-UNESP, Araçatuba, Brazil.
dc.format.extent391-394
dc.identifierhttp://dx.doi.org/10.1016/j.prevetmed.2015.08.016
dc.identifier.citationPreventive Veterinary Medicine, v. 121, n. 3-4, p. 391-394, 2015.
dc.identifier.doi10.1016/j.prevetmed.2015.08.016
dc.identifier.issn1873-1716
dc.identifier.lattes0903513897615274
dc.identifier.orcid0000-0003-0063-5172
dc.identifier.pubmed26342791
dc.identifier.urihttp://hdl.handle.net/11449/131622
dc.language.isoeng
dc.publisherElsevier B. V.
dc.relation.ispartofPreventive Veterinary Medicine
dc.rights.accessRightsAcesso restrito
dc.sourcePubMed
dc.subjectCryptosporidiosisen
dc.subjectDiarrheaen
dc.subjectIiaa14g2r1en
dc.subjectIiaa15g2r1en
dc.subjectOocystsen
dc.subjectGp60en
dc.titleFirst genetic identification of Cryptosporidium parvum subtype IIaA14G2R1in beef cattle in Brazilen
dc.typeArtigo
dcterms.rightsHolderElsevier B. V.
dspace.entity.typePublication
unesp.author.lattes0903513897615274
unesp.author.orcid0000-0003-0063-5172[9]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatubapt
unesp.departmentClínica, Cirurgia e Reprodução Animal - FMVApt

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Araçatuba - FMVA - Faculdade de Medicina Veterinária