An easy way to detect dengue virus using nanoparticle-antibody conjugates

Basso, Caroline R. [UNESP]; Tozato, Claudia C. [UNESP]; Crulhas, Bruno P. [UNESP]; Castro, Gustavo R. [UNESP]; Junior, João Pessoa A. [UNESP]; Pedrosa, Valber A. [UNESP]

doi:10.1016/j.virol.2017.10.001

An easy way to detect dengue virus using nanoparticle-antibody conjugates

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External sources

http://dx.doi.org/10.1016/j.virol.2017.10.001

External sources

http://dx.doi.org/10.1016/j.virol.2017.10.001

Date

2018-01-01

Authors

Basso, Caroline R.

Tozato, Claudia C.

Crulhas, Bruno P.

Castro, Gustavo R.

Junior, João Pessoa A.

Pedrosa, Valber A.

Type

Article

Access right

Acesso aberto

Files

External sources

http://dx.doi.org/10.1016/j.virol.2017.10.001

External sources

http://dx.doi.org/10.1016/j.virol.2017.10.001

Abstract

The aim of the present research is to propose a new method based on localized surface plasmon resonance (LSPR) for fast dengue virus detection. A pool with four dengue serotypes (DENV-1, -2, -3, -4) was detected through antigen-antibody binding using gold nanoparticles (AuNPs) as signaling antibody carriers. Such result was confirmed through surface plasmon resonance (SPR), transmission electron microcopy (TEM), and dynamic light scattering (DLS) techniques. The limit of detection was calculated for TCID50 107 demonstrating a linear correlation between viral concentration and number of cells with an r2 value of > 0.993. The assay presented good sensibility and reproducibility of results and the negative controls were not mistakenly detected. This design requires no pretreatment or high trained person. In the future, it can be used in commercial antibody detection kits.