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An easy way to detect dengue virus using nanoparticle-antibody conjugates

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dc.contributor.authorBasso, Caroline R. [UNESP]
dc.contributor.authorTozato, Claudia C. [UNESP]
dc.contributor.authorCrulhas, Bruno P. [UNESP]
dc.contributor.authorCastro, Gustavo R. [UNESP]
dc.contributor.authorJunior, João Pessoa A. [UNESP]
dc.contributor.authorPedrosa, Valber A. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:15:21Z
dc.date.available2018-12-11T17:15:21Z
dc.date.issued2018-01-01
dc.description.abstractThe aim of the present research is to propose a new method based on localized surface plasmon resonance (LSPR) for fast dengue virus detection. A pool with four dengue serotypes (DENV-1, -2, -3, -4) was detected through antigen-antibody binding using gold nanoparticles (AuNPs) as signaling antibody carriers. Such result was confirmed through surface plasmon resonance (SPR), transmission electron microcopy (TEM), and dynamic light scattering (DLS) techniques. The limit of detection was calculated for TCID50 107 demonstrating a linear correlation between viral concentration and number of cells with an r2 value of > 0.993. The assay presented good sensibility and reproducibility of results and the negative controls were not mistakenly detected. This design requires no pretreatment or high trained person. In the future, it can be used in commercial antibody detection kits.en
dc.description.affiliationDepartment of Chemistry and Biochemistry Institute of Bioscience UNESP-Botucatu
dc.description.affiliationDepartment of Microbiology and Immunology Institute of Bioscience UNESP-Botucatu
dc.description.affiliationUnespDepartment of Chemistry and Biochemistry Institute of Bioscience UNESP-Botucatu
dc.description.affiliationUnespDepartment of Microbiology and Immunology Institute of Bioscience UNESP-Botucatu
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2016/159191-8
dc.description.sponsorshipIdCNPq: 304581/2016-0
dc.format.extent85-90
dc.identifierhttp://dx.doi.org/10.1016/j.virol.2017.10.001
dc.identifier.citationVirology, v. 513, p. 85-90.
dc.identifier.doi10.1016/j.virol.2017.10.001
dc.identifier.file2-s2.0-85031294670.pdf
dc.identifier.issn1096-0341
dc.identifier.issn0042-6822
dc.identifier.scopus2-s2.0-85031294670
dc.identifier.urihttp://hdl.handle.net/11449/175336
dc.language.isoeng
dc.relation.ispartofVirology
dc.relation.ispartofsjr1,728
dc.rights.accessRightsAcesso abertopt
dc.sourceScopus
dc.subjectBiosensor
dc.subjectFast detection
dc.subjectInfectious diagnostics
dc.titleAn easy way to detect dengue virus using nanoparticle-antibody conjugatesen
dc.typeArtigopt
dspace.entity.typePublication
relation.isOrgUnitOfPublicationbc74a1ce-4c4c-4dad-8378-83962d76c4fd
relation.isOrgUnitOfPublicationab63624f-c491-4ac7-bd2c-767f17ac838d
relation.isOrgUnitOfPublication.latestForDiscoverybc74a1ce-4c4c-4dad-8378-83962d76c4fd
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Química, Araraquarapt
unesp.departmentMicrobiologia e Imunologia - IBBpt
unesp.departmentBioquímica e Tecnologia - IQpt

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Araraquara - IQAR - Instituto de Química
Botucatu - IBB - Instituto de Biociências