Publicação: Effects of light-curing time on the cytotoxicity of a restorative composite resin on odontoblast-like cells
dc.contributor.author | Fabio Aranha, Andreza Maria | |
dc.contributor.author | Aparecida Giro, Elisa Maria [UNESP] | |
dc.contributor.author | Hebling, Josimeri [UNESP] | |
dc.contributor.author | Rosetti Lessa, Fernanda Campos | |
dc.contributor.author | de Souza Costa, Carlos Alberto [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Universidade de São Paulo (USP) | |
dc.contributor.institution | Vila Velha Univ Ctr | |
dc.date.accessioned | 2013-09-30T18:32:34Z | |
dc.date.accessioned | 2014-05-20T13:44:49Z | |
dc.date.available | 2013-09-30T18:32:34Z | |
dc.date.available | 2014-05-20T13:44:49Z | |
dc.date.issued | 2010-09-01 | |
dc.description.abstract | This in vitro study evaluated the cytotoxicity of an experimental restorative composite resin subjected to different light-curing regimens. Methods: Forty round-shaped specimens were prepared and randomly assigned to four experimental groups (n=10), as follows: in Group 1, no light-curing; in Groups 2, 3 and 4, the composite resin specimens were light-cured for 20, 40 or 60 s, respectively. In Group 5, filter paper discs soaked in 5 mu L PBS were used as negative controls. The resin specimens and paper discs were placed in wells of 24-well plates in which the odontoblast-like cells MDPC-23 (30,000 cells/cm(2)) were plated and incubated in a humidified incubator with 5% CO(2) and 95% air at 37 degrees C for 72 h. The cytotoxicity was evaluated by the cell metabolism (MTT assay) and cell morphology (SEM). The data were analyzed statistically by Kruskal-Wallis and Mann-Whitney tests (p<0.05). Results: In G1, cell metabolism decreased by 86.2%, indicating a severe cytotoxicity of the non-light-cured composite resin. on the other hand, cell metabolism decreased by only 13.3% and 13.5% in G2 and G3, respectively. No cytotoxic effects were observed in G4 and G5. In G1, only a few round-shaped cells with short processes on their cytoplasmic membrane were observed. In the other experimental groups as well as in control group, a number of spindle-shaped cells with long cytoplasmic processes were found. Conclusion: Regardless of the photoactivation time used in the present investigation, the experimental composite resin presented mild to no toxic effects to the odontoblast-like MDPC-23 cells. However, intense cytotoxic effects occurred when no light-curing was performed. | en |
dc.description.affiliation | São Paulo State Univ, Araraquara Dent Sch, Discipline Pathol, Dept Physiol & Pathol, Araraquara, SP, Brazil | |
dc.description.affiliation | Univ São Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP, Brazil | |
dc.description.affiliation | São Paulo State Univ, Araraquara Dent Sch, Dept Orthodont & Pediat Dent, Discipline Pediat Dent, Araraquara, SP, Brazil | |
dc.description.affiliation | Vila Velha Univ Ctr, Discipline Pathol, Vitoria, ES, Brazil | |
dc.description.affiliationUnesp | São Paulo State Univ, Araraquara Dent Sch, Discipline Pathol, Dept Physiol & Pathol, Araraquara, SP, Brazil | |
dc.description.affiliationUnesp | São Paulo State Univ, Araraquara Dent Sch, Dept Orthodont & Pediat Dent, Discipline Pediat Dent, Araraquara, SP, Brazil | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description.sponsorshipId | FAPESP: 08/08424-6 | |
dc.description.sponsorshipId | CNPq: 301029/2007-5 | |
dc.format.extent | 461-466 | |
dc.identifier | http://dx.doi.org/10.1590/S1678-77572010000500006 | |
dc.identifier.citation | Journal of Applied Oral Science. Bauru-sp: Univ São Paulo Fac Odontologia Bauru, v. 18, n. 5, p. 461-466, 2010. | |
dc.identifier.file | S1678-77572010000500006-en.pdf | |
dc.identifier.issn | 1678-7757 | |
dc.identifier.lattes | 0097031682063652 | |
dc.identifier.lattes | 4517484241515548 | |
dc.identifier.scielo | S1678-77572010000500006 | |
dc.identifier.uri | http://hdl.handle.net/11449/15718 | |
dc.identifier.wos | WOS:000284590700006 | |
dc.language.iso | eng | |
dc.publisher | Universidade de São Paulo (USP), Faculdade de Odontologia de Bauru | |
dc.relation.ispartof | Journal of Applied Oral Science | |
dc.relation.ispartofjcr | 1.709 | |
dc.relation.ispartofsjr | 0,645 | |
dc.rights.accessRights | Acesso aberto | pt |
dc.source | Web of Science | |
dc.subject | Dental materials | en |
dc.subject | Cytotoxicity | en |
dc.subject | Dental pulp | en |
dc.subject | Cultured cells | en |
dc.title | Effects of light-curing time on the cytotoxicity of a restorative composite resin on odontoblast-like cells | en |
dc.type | Resumo | pt |
dcterms.license | http://www.scielo.br/revistas/jaos/paboutj.htm | |
dcterms.rightsHolder | Univ São Paulo Fac Odontologia Bauru | |
dspace.entity.type | Publication | |
relation.isDepartmentOfPublication | b3ba3d9c-022e-4521-8805-0bcceea7372e | |
relation.isDepartmentOfPublication.latestForDiscovery | b3ba3d9c-022e-4521-8805-0bcceea7372e | |
relation.isOrgUnitOfPublication | ca4c0298-cd82-48ee-a9c8-c97704bac2b0 | |
relation.isOrgUnitOfPublication.latestForDiscovery | ca4c0298-cd82-48ee-a9c8-c97704bac2b0 | |
unesp.author.lattes | 0097031682063652 | |
unesp.author.lattes | 4517484241515548[5] | |
unesp.author.orcid | 0000-0002-2085-2951[2] | |
unesp.author.orcid | 0000-0002-7455-6867[5] | |
unesp.campus | Universidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquara | pt |
unesp.department | Clínica Infantil - FOAR | pt |
unesp.department | Fisiologia e Patologia - FOAR | pt |
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