Logotipo do repositório
 

Publicação:
Effects of light-curing time on the cytotoxicity of a restorative composite resin on odontoblast-like cells

dc.contributor.authorFabio Aranha, Andreza Maria
dc.contributor.authorAparecida Giro, Elisa Maria [UNESP]
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorRosetti Lessa, Fernanda Campos
dc.contributor.authorde Souza Costa, Carlos Alberto [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionVila Velha Univ Ctr
dc.date.accessioned2013-09-30T18:32:34Z
dc.date.accessioned2014-05-20T13:44:49Z
dc.date.available2013-09-30T18:32:34Z
dc.date.available2014-05-20T13:44:49Z
dc.date.issued2010-09-01
dc.description.abstractThis in vitro study evaluated the cytotoxicity of an experimental restorative composite resin subjected to different light-curing regimens. Methods: Forty round-shaped specimens were prepared and randomly assigned to four experimental groups (n=10), as follows: in Group 1, no light-curing; in Groups 2, 3 and 4, the composite resin specimens were light-cured for 20, 40 or 60 s, respectively. In Group 5, filter paper discs soaked in 5 mu L PBS were used as negative controls. The resin specimens and paper discs were placed in wells of 24-well plates in which the odontoblast-like cells MDPC-23 (30,000 cells/cm(2)) were plated and incubated in a humidified incubator with 5% CO(2) and 95% air at 37 degrees C for 72 h. The cytotoxicity was evaluated by the cell metabolism (MTT assay) and cell morphology (SEM). The data were analyzed statistically by Kruskal-Wallis and Mann-Whitney tests (p<0.05). Results: In G1, cell metabolism decreased by 86.2%, indicating a severe cytotoxicity of the non-light-cured composite resin. on the other hand, cell metabolism decreased by only 13.3% and 13.5% in G2 and G3, respectively. No cytotoxic effects were observed in G4 and G5. In G1, only a few round-shaped cells with short processes on their cytoplasmic membrane were observed. In the other experimental groups as well as in control group, a number of spindle-shaped cells with long cytoplasmic processes were found. Conclusion: Regardless of the photoactivation time used in the present investigation, the experimental composite resin presented mild to no toxic effects to the odontoblast-like MDPC-23 cells. However, intense cytotoxic effects occurred when no light-curing was performed.en
dc.description.affiliationSão Paulo State Univ, Araraquara Dent Sch, Discipline Pathol, Dept Physiol & Pathol, Araraquara, SP, Brazil
dc.description.affiliationUniv São Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP, Brazil
dc.description.affiliationSão Paulo State Univ, Araraquara Dent Sch, Dept Orthodont & Pediat Dent, Discipline Pediat Dent, Araraquara, SP, Brazil
dc.description.affiliationVila Velha Univ Ctr, Discipline Pathol, Vitoria, ES, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Araraquara Dent Sch, Discipline Pathol, Dept Physiol & Pathol, Araraquara, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Araraquara Dent Sch, Dept Orthodont & Pediat Dent, Discipline Pediat Dent, Araraquara, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 08/08424-6
dc.description.sponsorshipIdCNPq: 301029/2007-5
dc.format.extent461-466
dc.identifierhttp://dx.doi.org/10.1590/S1678-77572010000500006
dc.identifier.citationJournal of Applied Oral Science. Bauru-sp: Univ São Paulo Fac Odontologia Bauru, v. 18, n. 5, p. 461-466, 2010.
dc.identifier.fileS1678-77572010000500006-en.pdf
dc.identifier.issn1678-7757
dc.identifier.lattes0097031682063652
dc.identifier.lattes4517484241515548
dc.identifier.scieloS1678-77572010000500006
dc.identifier.urihttp://hdl.handle.net/11449/15718
dc.identifier.wosWOS:000284590700006
dc.language.isoeng
dc.publisherUniversidade de São Paulo (USP), Faculdade de Odontologia de Bauru
dc.relation.ispartofJournal of Applied Oral Science
dc.relation.ispartofjcr1.709
dc.relation.ispartofsjr0,645
dc.rights.accessRightsAcesso abertopt
dc.sourceWeb of Science
dc.subjectDental materialsen
dc.subjectCytotoxicityen
dc.subjectDental pulpen
dc.subjectCultured cellsen
dc.titleEffects of light-curing time on the cytotoxicity of a restorative composite resin on odontoblast-like cellsen
dc.typeResumopt
dcterms.licensehttp://www.scielo.br/revistas/jaos/paboutj.htm
dcterms.rightsHolderUniv São Paulo Fac Odontologia Bauru
dspace.entity.typePublication
relation.isDepartmentOfPublicationb3ba3d9c-022e-4521-8805-0bcceea7372e
relation.isDepartmentOfPublication.latestForDiscoveryb3ba3d9c-022e-4521-8805-0bcceea7372e
relation.isOrgUnitOfPublicationca4c0298-cd82-48ee-a9c8-c97704bac2b0
relation.isOrgUnitOfPublication.latestForDiscoveryca4c0298-cd82-48ee-a9c8-c97704bac2b0
unesp.author.lattes0097031682063652
unesp.author.lattes4517484241515548[5]
unesp.author.orcid0000-0002-2085-2951[2]
unesp.author.orcid0000-0002-7455-6867[5]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquarapt
unesp.departmentClínica Infantil - FOARpt
unesp.departmentFisiologia e Patologia - FOARpt

Arquivos

Pacote Original

Agora exibindo 1 - 1 de 1
Carregando...
Imagem de Miniatura
Nome:
S1678-77572010000500006-en.pdf
Tamanho:
227.36 KB
Formato:
Adobe Portable Document Format
Descrição:

Licença do Pacote

Agora exibindo 1 - 2 de 2
Carregando...
Imagem de Miniatura
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Carregando...
Imagem de Miniatura
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição: