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Characterization of novel calcium hydroxide-mediated highly porous chitosan-calcium scaffolds for potential application in dentin tissue engineering

dc.contributor.authorSoares, Diana Gabriela
dc.contributor.authorBordini, Ester Alves Ferreira [UNESP]
dc.contributor.authorCassiano, Fernanda Balestrero [UNESP]
dc.contributor.authorBronze-Uhle, Erika Soares
dc.contributor.authorPacheco, Leandro Edgar
dc.contributor.authorZabeo, Giovana
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorLisboa-Filho, Paulo Noronha [UNESP]
dc.contributor.authorBottino, Marco Cicero
dc.contributor.authorde Souza Costa, Carlos Alberto [UNESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Michigan
dc.date.accessioned2020-12-12T01:56:53Z
dc.date.available2020-12-12T01:56:53Z
dc.date.issued2020-08-01
dc.description.abstractThe aim of this study was to develop a highly porous calcium-containing chitosan scaffold suitable for dentin regeneration. A calcium hydroxide (Ca[OH]2) suspension was used to modulate the degree of porosity and chemical composition of chitosan scaffolds. The chitosan solution concentration and freezing protocol were adjusted to optimize the porous architecture using the phase-separation technique. Scanning electron microscopy/energy-dispersive spectroscopy demonstrated the fabrication of a highly porous calcium-linked chitosan scaffold (CH-Ca), with a well-organized and interconnected porous network. Scaffolds were cross-linked on glutaraldehyde (GA) vapor. Following a 28-day incubation in water, cross-linked CH scaffold had no changes on humid mass, and CH-Ca featured a controlled degradability profile since the significant humid mass loss was observed only after 21 (26.0%) and 28 days (42.2%). Fourier-transform infrared spectroscopy indicated the establishment of Schiff base on cross-linked scaffolds, along with calcium complexation for CH-Ca. Cross-linked CH-Ca scaffold featured a sustained Ca2+ release up to 21 days in a humid environment. This porous and stable architecture allowed for human dental pulp cells (HDPCs) to spread throughout the scaffold, with cells exhibiting a widely stretched cytoplasm; whereas, the cells seeded onto CH scaffold were organized in clusters. HDPCs seeded onto CH-Ca featured significantly higher ALP activity, and gene expressions for ALP, Col1, DMP-1, and DSPP in comparison to CH, leading to a significant 3.5 times increase in calcium-rich matrix deposition. In sum, our findings suggest that CH-Ca scaffolds are attractive candidates for creating a highly porous and bioactive substrate for dentin tissue engineering.en
dc.description.affiliationDepartment of Operative Dentistry Endodontics and Dental Materials Bauru School of Dentistry Sao Paulo University - USP
dc.description.affiliationDepartment of Physiology and Pathology Araraquara School of Dentistry Univ. Estadual Paulista - UNESP
dc.description.affiliationDepartment of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry Univ. Estadual Paulista – UNESP
dc.description.affiliationDepartment of Physics School of Sciences Univ. Estadual Paulista – UNESP
dc.description.affiliationDepartment of Cariology Restorative Sciences Endodontics School of Dentistry University of Michigan
dc.description.affiliationUnespDepartment of Physiology and Pathology Araraquara School of Dentistry Univ. Estadual Paulista - UNESP
dc.description.affiliationUnespDepartment of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry Univ. Estadual Paulista – UNESP
dc.description.affiliationUnespDepartment of Physics School of Sciences Univ. Estadual Paulista – UNESP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdFAPESP: 2016/15674-5
dc.format.extent2546-2559
dc.identifierhttp://dx.doi.org/10.1002/jbm.b.34586
dc.identifier.citationJournal of Biomedical Materials Research - Part B Applied Biomaterials, v. 108, n. 6, p. 2546-2559, 2020.
dc.identifier.doi10.1002/jbm.b.34586
dc.identifier.issn1552-4981
dc.identifier.issn1552-4973
dc.identifier.scopus2-s2.0-85079454970
dc.identifier.urihttp://hdl.handle.net/11449/200070
dc.language.isoeng
dc.relation.ispartofJournal of Biomedical Materials Research - Part B Applied Biomaterials
dc.sourceScopus
dc.subjectcalcium hydroxide
dc.subjectchitosan
dc.subjectdental pulp
dc.subjectdentin
dc.subjectporous scaffolds
dc.titleCharacterization of novel calcium hydroxide-mediated highly porous chitosan-calcium scaffolds for potential application in dentin tissue engineeringen
dc.typeArtigopt
dspace.entity.typePublication
relation.isDepartmentOfPublicationb3ba3d9c-022e-4521-8805-0bcceea7372e
relation.isDepartmentOfPublication.latestForDiscoveryb3ba3d9c-022e-4521-8805-0bcceea7372e
relation.isOrgUnitOfPublicationca4c0298-cd82-48ee-a9c8-c97704bac2b0
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unesp.author.lattes4517484241515548[10]
unesp.author.orcid0000-0002-1485-6104[1]
unesp.author.orcid0000-0002-7734-4069[8]
unesp.author.orcid0000-0001-8740-2464[9]
unesp.author.orcid0000-0002-7455-6867[10]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquarapt
unesp.departmentClínica Infantil - FOARpt
unesp.departmentFisiologia e Patologia - FOARpt

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