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Role of roscovitine and IBMX on kinetics of nuclear and cytoplasmic maturation of bovine oocytes in vitro

dc.contributor.authorBarretto, L. S. S.
dc.contributor.authorCastro, V. S. D. Caiado
dc.contributor.authorGarcia, J. M.
dc.contributor.authorMingoti, Gisele Zoccal [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:25:56Z
dc.date.available2014-05-20T15:25:56Z
dc.date.issued2007-05-01
dc.description.abstractThe 3-isobutyl-1-methylxanthine (IBMX) is able to prevent resumption of meiosis by maintaining elevated cyclic AMP (cAMP) concentrations in the oocyte, and roscovitine, a purine known to specifically inhibit MPF kinase activity, maintains bovine oocytes at the germinal vesicle (GV) stage. The present study was conducted to analyze whether cytoplasmic maturation (examined by the pattern of cortical granule (CG) distribution) of bovine oocytes is improved during meiotic arrest with IBMX and roscovitine. Oocytes were matured in vitro in a 10% Knockout(SR) supplemented TCM-199 medium (Control) with either 0.5 mM IBMX or 25 mu M roscovitine (ROSC). Oocytes were stained with fluorescein isothiocyanate conjugated Lens culinaris agglutinin (FITC-LCA) for CG evaluation and with Hoechst 33342 for nuclear stage assessment. At 16 h of culture, the percentage of oocytes remaining in the GV stage was higher (P < 0.05) in the ROSC group (32.41%) compared with the Control and IBMX groups (8.61% and 9.73%, respectively). At 24h of culture, progression of meiosis to M II stage was retarded (P < 0.05) in the ROSC group (24.05%) compared to the Control (60.20%), whereas the IBMX group (33.88%) showed no significant difference to the other two groups. At 16h of maturation, the proportion of oocytes with CG in clusters (immature cytoplasm) was similar between the groups, as was the percentage of peripheral CG (mature) at 24h of maturation. The results of the present study demonstrated that the meiotic inhibitors IBMX and roscovitine delay the progression of nuclear maturation without affecting cytoplasmic maturation, assessed by the analysis of CG repositioning. (c) 2006 Elsevier B.V. All rights reserved.en
dc.description.affiliationUNESP, Sch Vet Med, Dept Anim Hlth, BR-16050680 Aracatuba, SP, Brazil
dc.description.affiliationUNESP, Sch Agr & Vet Sci, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationUnespUNESP, Sch Vet Med, Dept Anim Hlth, BR-16050680 Aracatuba, SP, Brazil
dc.description.affiliationUnespUNESP, Sch Agr & Vet Sci, BR-14884900 Jaboticabal, SP, Brazil
dc.format.extent202-207
dc.identifierhttp://dx.doi.org/10.1016/j.anireprosci.2006.06.001
dc.identifier.citationAnimal Reproduction Science. Amsterdam: Elsevier B.V., v. 99, n. 1-2, p. 202-207, 2007.
dc.identifier.doi10.1016/j.anireprosci.2006.06.001
dc.identifier.issn0378-4320
dc.identifier.lattes4191951764334766
dc.identifier.orcid0000-0002-3059-4458
dc.identifier.urihttp://hdl.handle.net/11449/36251
dc.identifier.wosWOS:000245371600020
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofAnimal Reproduction Science
dc.relation.ispartofjcr1.647
dc.relation.ispartofsjr0,704
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcattlept
dc.subjectoocytept
dc.subjectmaturationpt
dc.subjectIBMXpt
dc.subjectroscovitinept
dc.subjectknockoutpt
dc.titleRole of roscovitine and IBMX on kinetics of nuclear and cytoplasmic maturation of bovine oocytes in vitroen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dspace.entity.typePublication
unesp.author.lattes4191951764334766
unesp.author.orcid0000-0002-3059-4458[4]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatubapt
unesp.departmentApoio, Produção e Saúde Animal - FMVApt

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