Publicação:
Antimicrobial potential, phytochemical profile, cytotoxic and genotoxic screening of sedum praealtum a. DC. (balsam)

dc.contributor.authorGomes Boriollo, Marcelo Fabiano
dc.contributor.authorMarques, Milene Bueno
dc.contributor.authorDa Silva, Thaísla Andrielle
dc.contributor.authorDa Silva, Jeferson Júnior
dc.contributor.authorDias, Reigson Alves
dc.contributor.authorSilva Filho, Thyago Henrique Neves
dc.contributor.authorRibeiro Melo, Isadora Letícia
dc.contributor.authorDos Santos Dias, Carlos Tadeu
dc.contributor.authorDe Carvalho Bernardo, Wagner Luís [UNESP]
dc.contributor.authorDe Mello Silva Oliveira, Nelma
dc.contributor.authorPeters, Vera Maria
dc.contributor.authorHöfling, José Francisco
dc.contributor.authorPalomari Spolidorio, Denise Madalena [UNESP]
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversity of Alfenas (UNIFENAS)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFederal University of Juiz de Fora (UFJF)
dc.date.accessioned2020-12-12T02:10:22Z
dc.date.available2020-12-12T02:10:22Z
dc.date.issued2020-04-29
dc.description.abstractBackground: Sedum praealtum has been used for a long time in traditional medicine as an analgesic and antiinflammatory agent. Its beneficial effects have been known since ancient times, when Latinos used it to treat sore and swollen eyes. This research evaluated the antimicrobial potential, the cytotoxic and genotoxic effects, and some chromatographic profiles of the hydroethanolic extract of leaves, stems and roots of S. praealtum. Methods: The antimicrobial activities were carried out by broth microdilution and agar diffusion. In vitro cytotoxicity was evaluated by cell cultures of Aedes albopictus and the selectivity index (SI) was estimated: SI=CI50/MIC. Genotoxic and systemic toxic effects of S. praealtum leaves were analyzed by micronucleus assay in mice bone marrow. Chromatographic profiles and mass spectra were investigated by GC-MS. Results: Gram-positive (B. subtilis, B. cereus, M. luteus, E. faecalis and S. aureus) and gram-negative (E. coli, E. aerogenes, S. marcescens, P. aeruginosa, P. mirabilis and S. typhimurium) bacteria exhibited MICs ranging from 12.5–50 and 0–50 mg/ ml, respectively. Sedum praealtum showed no efficacy against M. tuberculosis and M. bovis. Cytotoxicity (CI50) of S. praealtum was 4.22 and 5.96 mg/ml for leaves and stems, respectively, while its roots showed no cytotoxicity. Micronucleated polychromatic erythrocytes (MNPCEs) analyzes showed no differences between treatment doses (0.5–2 g/kg) and negative control (NaCl), but the PCE/NCE ratio (polychromatic erythrocyte/normochromatic erythrocyte) showed significant differences. Phytochemical screening identified thirteen compounds in the leaves, stems and roots of S. praealtum potentially associated with their biological activities. Conclusions: This research comprises a first scientific study on genotoxicity, cytotoxicity and antimicrobial effects of S. praealtum (Balsam), and it provides an initial theoretical foundation for its comprehensive use. Results showed antibacterial action of S. praealtum against gram-positive bacteria and some gram-negative species (depending on the plant anatomical part), but ineffective antimycobacterial action. However, S. praealtum leaves and stems display potential cytotoxicity, contributing to the SI < 1 values. In addition, S. praealtum leaves exhibit no clastogenic and/or aneugenic effects, but it has systemic toxicity dose-independent.en
dc.description.affiliationLaboratory of Microbiology and Immunology Department of Oral Diagnosis Dental School of Piracicaba State University of Campinas (FOP/UNICAMP)
dc.description.affiliationCenter for Research and Postgraduate Studies in Animal Science Pathology and Animal Pharmacology Area University of Alfenas (UNIFENAS)
dc.description.affiliationLaboratory of Pharmacogenetics and Molecular Biology Faculty of Medical Sciences University of Alfenas (UNIFENAS)
dc.description.affiliationDepartment of Exact Sciences College of Agriculture University of São Paulo (ESALQ/USP)
dc.description.affiliationLaboratory of Oral Microbiology and Immunology Department of Physiology and Oral Pathology Araraquara School of Dentistry São Paulo State University (FOAr/UNESP)
dc.description.affiliationReproductive Biology Center Federal University of Juiz de Fora (UFJF)
dc.description.affiliationUnespLaboratory of Oral Microbiology and Immunology Department of Physiology and Oral Pathology Araraquara School of Dentistry São Paulo State University (FOAr/UNESP)
dc.identifierhttp://dx.doi.org/10.1186/s12906-020-02915-6
dc.identifier.citationBMC Complementary Medicine and Therapies, v. 20, n. 1, 2020.
dc.identifier.doi10.1186/s12906-020-02915-6
dc.identifier.issn2662-7671
dc.identifier.scopus2-s2.0-85086083329
dc.identifier.urihttp://hdl.handle.net/11449/200580
dc.language.isoeng
dc.relation.ispartofBMC Complementary Medicine and Therapies
dc.sourceScopus
dc.subjectAntimicrobial susceptibility
dc.subjectCytotoxicity
dc.subjectGenotoxicity
dc.subjectPhytochemical screening
dc.subjectSedum praealtum a. DC
dc.titleAntimicrobial potential, phytochemical profile, cytotoxic and genotoxic screening of sedum praealtum a. DC. (balsam)en
dc.typeArtigo
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquarapt
unesp.departmentFisiologia e Patologia - FOARpt

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