Antimicrobial Resistance in Chicken Meat: Comparing Salmonella, Escherichia coli, and Enterococcus from Conventional and Antibiotic-Free Productions

Abstract

Chicken meat production is a critical component of the global protein supply, significantly influenced by rearing advancements, including the use of antimicrobial agents. However, the pervasive use of antibiotics has raised concerns regarding the occurrence of antimicrobial resistance (AMR). This study examined the prevalence and AMR profiles of <i>Salmonella</i> spp., <i>Escherichia coli</i>, and <i>Enterococcus</i> spp. in chicken meat from conventional and antibiotic-free (ABF) production chains. A total of 284 samples were analyzed for <i>Salmonella</i> spp. and <i>E. coli</i>, while 164 samples were tested for <i>Enterococcus</i> spp. From that, 143 were from conventional production chains and 141 were from ABF chains. The results indicated a 10.9% prevalence of <i>Salmonella</i> spp., 22.1% for <i>E. coli</i>, and 93.9% for <i>Enterococcus</i> spp. Regarding production chains, the conventional chain had 18.2% of the isolates for <i>Salmonella</i> spp., 20.3% for <i>E. coli</i>, and 91.6% for <i>Enterococcus</i> spp., while the ABF chain had 3.5% of the isolates for <i>Salmonella</i> spp., 24.1% for <i>E. coli</i>, and 96.3% for <i>Enterococcus</i> spp. In terms of AMR, 86.1% of the <i>Salmonella</i> spp. isolates that underwent the disk diffusion test were resistant to at least one antibiotic tested, 95.1% of <i>E. coli</i>, and 88.4% of <i>Enterococcus</i> spp. Notably, carbapenem resistance was detected in <i>Salmonella</i> spp., with 2.3% of isolates being resistant to imipenem, while resistance to vancomycin and linezolid was detected in <i>Enterococcus</i> spp., all of which are critically important antimicrobials. Comparisons between these production chains revealed significant differences in antibiotic resistance patterns in <i>Salmonella</i> spp. for two antibiotics, amoxicillin/clavulanic acid and nitrofurantoin, while no differences were observed in <i>E. coli</i>. For <i>Enterococcus</i> spp., resistance varied for three antibiotics: streptomycin, penicillin, and tetracycline. For all other antibiotics tested, the resistance profiles were consistent across both conventional and ABF production chains. Multidrug resistance (MDR) was observed in 90.7% of <i>Salmonella</i> spp. isolates, 42.9% of <i>E. coli</i> isolates, and 12.0% of <i>Enterococcus</i> spp. isolates. Statistically significant differences were noted in MDR prevalence between production chains, with conventional production systems exhibiting higher levels of MDR isolates compared to ABF systems. These findings underscore the need for targeted AMR control strategies that consider the complexity of resistance dynamics across production systems.