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A new immunoassay of hybrid nanomater conjugated to aptamers for the detection of dengue virus

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dc.contributor.authorBasso, Caroline R. [UNESP]
dc.contributor.authorCrulhas, Bruno P. [UNESP]
dc.contributor.authorMagro, Massimiliano
dc.contributor.authorVianello, Fabio
dc.contributor.authorPedrosa, Valber A. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Padua
dc.date.accessioned2019-10-06T15:31:33Z
dc.date.available2019-10-06T15:31:33Z
dc.date.issued2019-05-15
dc.description.abstractA new immunosensor using hybrid nanomaterials for the detection of dengue virus was demonstrated in this work. This immunosensor composed of nanoparticles of γ-Fe2O3(SAMN) modified with MPA- SAMN@MPA was characterized by FTIR spectroscopy, transmission electron microscopy,quartz crystal microbalance, UV–vis and LSPR technique. The binding of SAMN@MPA with AuNPs conjugated with aptamers(SAMN@MPA@AuNPs@aptamer) provides specific chemical bonds to four dengue serotypes. Colorimetric changes in the modification steps provided rapid visual detection of the virus without the use of equipment. Variations of aptamers concentrations 1.0-10.0 μM where the 3.0 μM aptamer concentration is sufficient to completely cover the surface of the modified AuNPs with an R2 value of> 0.99. This new proposed methodology presenting some advantages in relation to traditional detection methods such as time optimization and cost,can be used as a diagnostic method.en
dc.description.affiliationInstitute of Bioscience Department of Chemistry and Biochemistry UNESP
dc.description.affiliationDepartment of Comparative Biomedicine and Food Science University of Padua
dc.description.affiliationUnespInstitute of Bioscience Department of Chemistry and Biochemistry UNESP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent482-490
dc.identifierhttp://dx.doi.org/10.1016/j.talanta.2019.01.058
dc.identifier.citationTalanta, v. 197, p. 482-490.
dc.identifier.dimensionspub.1111490103
dc.identifier.doi10.1016/j.talanta.2019.01.058
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.orcid0000-0002-4644-004X
dc.identifier.orcid0000-0002-4874-7205
dc.identifier.orcid0000-0002-9950-7711
dc.identifier.orcid0000-0003-4445-7283
dc.identifier.orcid0000-0001-7104-2337
dc.identifier.pmid30771965
dc.identifier.scopus2-s2.0-85060475512
dc.identifier.urihttp://hdl.handle.net/11449/187289
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofTalanta
dc.rights.accessRightsAcesso abertopt
dc.sourceScopus
dc.sourceDimensions
dc.subjectDengue virus
dc.subjectHybrid nanomaterial
dc.subjectLocalized surface plasmon resonance
dc.titleA new immunoassay of hybrid nanomater conjugated to aptamers for the detection of dengue virusen
dc.typeArtigopt
dspace.entity.typePublication

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