Publicação: VEGF-C expression in oral cancer by neurotransmitter-induced activation of beta-adrenergic receptors
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2013-02-01
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Coorientador
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The aim of this study was to investigate the expression of vascular endothelial growth factor type C (VEGF-C) in oral squamous cell carcinoma (OSCC) cell lines through norepinephrine-induced activation of beta-adrenergic receptors. Human OSCC cell lines (SCC-9 and SCC-25) expressing beta-adrenergic receptors were stimulated with different concentrations of norepinephrine (0.1, 1, and 10 μM) and 1 μM of propranolol, and analyzed after 1, 6, and 24 h. VEGF-C gene expression and VEGF-C production in the cell supernatant were evaluated by real-time PCR and by ELISA, respectively. The results showed that beta-adrenergic receptor stimulation by different concentrations of norepinephrine or blocking by propranolol did not markedly alter VEGF-C expression by SCC-9 and SCC-25 cells. VEGF-C protein levels produced by oral malignant cell lines after stimulation with different norepinephrine concentrations or blocking with propranolol was statistically similar (p > 0.05) to those of the control group (nonstimulated OSCC cell lines). Our findings suggest that stimulation of beta-adrenergic receptors by means of norepinephrine does not seem to modulate the VEGF-C expression in OSCC cell lines. These findings reinforce the need for further studies in order to understand the responsiveness of oral cancer to beta-adrenergic receptor stimulation or blockage, especially with regard to VEGF-C production. © 2012 International Society of Oncology and BioMarkers (ISOBM).
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Adrenergic receptors, Oral cancer, VEGF, beta adrenergic receptor, noradrenalin, propranolol, vasculotropin C, cancer cell, cancer cell culture, cell line SCC 25, cell line SCC 9, controlled study, enzyme activation, enzyme activity, enzyme synthesis, human, human cell, in vitro study, mouth squamous cell carcinoma, priority journal, protein analysis, protein expression, protein function, Adrenergic beta-Antagonists, Carcinoma, Squamous Cell, Cell Line, Tumor, Humans, Mouth Neoplasms, Neurotransmitter Agents, Norepinephrine, Propranolol, Receptors, Adrenergic, beta, Vascular Endothelial Growth Factor C
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Inglês
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Tumor Biology, v. 34, n. 1, p. 139-143, 2013.
