Isolation and in vitro hydrolysis of lentil protein fractions by trypsin
dc.contributor.author | Neves, Valdir Augusto [UNESP] | |
dc.contributor.author | Lourenco, E. J. | |
dc.contributor.author | daSilva, M. A. | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2014-05-20T15:21:39Z | |
dc.date.available | 2014-05-20T15:21:39Z | |
dc.date.issued | 1996-09-01 | |
dc.description.abstract | The albumin and globulin fractions from lentil seeds were isolated and characterised by gel filtration. The latter was shown to be homogeneous and the former heterogeneous on PAGE. The aminoacid analysis revealed high values of amidic amino acids for both fractions with great differences in the sulphur-containing amino acids. Native albumin, globulin and salt-soluble proteins were markedly resistant to trypsin hydrolysis compared to casein. The SDS-PAGE of native salt-soluble proteins indicated that the globulin fragments (20 to 30 kD) were slowly digested in the presence of albumin. The heating increased the hydrolysis of the proteins in the order: salt-soluble, albumin and globulin. The facilitated hydrolysis of the heated salt-soluble fraction seemed to be due to protein-protein interactions induced by heat. | en |
dc.description.affiliation | UNESP,FAC CIENCIAS FARMACEUT,DEPT ALIMENTOS & NUTR,ARARAQUARA,SP,BRAZIL | |
dc.description.affiliationUnesp | UNESP,FAC CIENCIAS FARMACEUT,DEPT ALIMENTOS & NUTR,ARARAQUARA,SP,BRAZIL | |
dc.format.extent | 238-242 | |
dc.identifier | http://www.scielo.org.ve/scielo.php?script=sci_issues&pid=0004-0622&lng=es&nrm=iso | |
dc.identifier.citation | Archivos Latinoamericanos de Nutricion. Caracas: Archivos Latinoamericanos Nutricion, v. 46, n. 3, p. 238-242, 1996. | |
dc.identifier.issn | 0004-0622 | |
dc.identifier.lattes | 4031319519910419 | |
dc.identifier.uri | http://hdl.handle.net/11449/32784 | |
dc.identifier.wos | WOS:A1996WP61600011 | |
dc.language.iso | eng | |
dc.publisher | Archivos Latinoamericanos Nutricion | |
dc.relation.ispartof | Archivos Latinoamericanos de Nutrición | |
dc.relation.ispartofjcr | 0.358 | |
dc.relation.ispartofsjr | 0,179 | |
dc.rights.accessRights | Acesso restrito | pt |
dc.source | Web of Science | |
dc.title | Isolation and in vitro hydrolysis of lentil protein fractions by trypsin | en |
dc.type | Artigo | pt |
dcterms.license | http://www.scielo.org.ve/revistas/alan/eaboutj.htm | |
dcterms.rightsHolder | Archivos Latinoamericanos Nutricion | |
dspace.entity.type | Publication | |
relation.isOrgUnitOfPublication | 95697b0b-8977-4af6-88d5-c29c80b5ee92 | |
relation.isOrgUnitOfPublication.latestForDiscovery | 95697b0b-8977-4af6-88d5-c29c80b5ee92 | |
unesp.author.lattes | 4031319519910419 | |
unesp.campus | Universidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquara | pt |
unesp.department | Alimentos e Nutrição - FCF | pt |
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