Publicação: Synthesis of the peptide Ac-Wahx-KTTKS and evaluation of the ability to induce in vitro collagen synthesis
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In this work, we report the synthesis of a peptide analogue of the KTTKS, termed Ac-Wahx-KTTKS and evaluate its cytotoxicity and role in biosynthesis of collagen for future application in skin aging. The peptide was obtained with purity higher than 97.5%. In the cytotoxicity assay, we observed non-toxic effects for Ac-WAhx-KTTKS at concentrations below 600 ôM for HaCaT and 500 ôM for HepG2 cells, respectively. After 24 and 48 h it was possible to observe significant changes in collagen synthesis in the groups treated with various concentrations of the peptide. In conclusion, the Ac-Wahx-KTTKS peptide increased collagen synthesis in fibroblasts by 80% and it is a promising candidate for improving skin aging.
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Ac-WAhx-KTTKS, Antiaging, Collagen biosynthesis, Cytotoxicity, Peptide synthesis, Skin functions.
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Inglês
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Protein and Peptide Letters, v. 23, n. 6, p. 544-547, 2016.
