Comparison of hexamethyidisilazane and critical point drying treatments for SEM analysis of anaerobic biofilms and granular sludge
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Undergraduate course
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Oxford University Press
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Article
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Abstract
We present a fast procedure for scanning electron microscopy (SEM) analysis in which hexamethyldisilazane (HMDS) solvent, instead of the critical point drying, is used to remove liquids from a microbiological specimen. The results indicate that the HMDS solvent is suitable for drying samples of anaerobic cells for examination by SEM and does not cause cell structure disruption.
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Keywords
anaerobic biofilms, critical point drying method, granular sludge, HMDS treatment, SEM analysis
Language
English
Citation
Journal of Electron Microscopy. Oxford: Oxford Univ Press, v. 52, n. 4, p. 429-433, 2003.




