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Comparison of hexamethyidisilazane and critical point drying treatments for SEM analysis of anaerobic biofilms and granular sludge

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dc.contributor.authorAraujo, J. C.
dc.contributor.authorTeran, F. C.
dc.contributor.authorOliveira, R. A.
dc.contributor.authorNour, EAA
dc.contributor.authorMontenegro, MAP
dc.contributor.authorCampos, JR
dc.contributor.authorVazoller, R. F.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2014-05-20T15:24:40Z
dc.date.available2014-05-20T15:24:40Z
dc.date.issued2003-01-01
dc.description.abstractWe present a fast procedure for scanning electron microscopy (SEM) analysis in which hexamethyldisilazane (HMDS) solvent, instead of the critical point drying, is used to remove liquids from a microbiological specimen. The results indicate that the HMDS solvent is suitable for drying samples of anaerobic cells for examination by SEM and does not cause cell structure disruption.en
dc.description.affiliationUniv São Paulo, Escola Engn Sao Carlos, Dept Hidraul & Saneamento, BR-13566590 Sao Carlos, SP, Brazil
dc.description.affiliationUniv São Paulo, Inst Ciências Biomed, Dept Microbiol, Lab Microbiol Ambiental, BR-05508900 São Paulo, Brazil
dc.description.affiliationUNESP, Fac Ciências Agr & Vet, Jaboticabal, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Fac Civil Engn, Campinas, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Ciências Agr & Vet, Jaboticabal, SP, Brazil
dc.format.extent429-433
dc.identifierhttp://dx.doi.org/10.1093/jmicro/52.4.429
dc.identifier.citationJournal of Electron Microscopy. Oxford: Oxford Univ Press, v. 52, n. 4, p. 429-433, 2003.
dc.identifier.doi10.1093/jmicro/52.4.429
dc.identifier.issn0022-0744
dc.identifier.urihttp://hdl.handle.net/11449/35237
dc.identifier.wosWOS:000185860700011
dc.language.isoeng
dc.publisherOxford University Press
dc.relation.ispartofJournal of Electron Microscopy
dc.rights.accessRightsAcesso restritopt
dc.sourceWeb of Science
dc.subjectanaerobic biofilmspt
dc.subjectcritical point drying methodpt
dc.subjectgranular sludgept
dc.subjectHMDS treatmentpt
dc.subjectSEM analysispt
dc.titleComparison of hexamethyidisilazane and critical point drying treatments for SEM analysis of anaerobic biofilms and granular sludgeen
dc.typeArtigopt
dcterms.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dcterms.rightsHolderOxford Univ Press
dspace.entity.typePublication
unesp.author.orcid0000-0002-5900-3308[3]
unesp.author.orcid0000-0002-6178-0878[4]
unesp.author.orcid0000-0003-2832-2848[3]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias