Regulation of ghrelin receptor by microbial and inflammatory signals in human osteoblasts
dc.contributor.author | Nokhbehsaim, Marjan | |
dc.contributor.author | Nogueira, Andressa Vilas Boas | |
dc.contributor.author | Memmert, Svenja | |
dc.contributor.author | Damanaki, Anna | |
dc.contributor.author | Eick, Sigrun | |
dc.contributor.author | Cirelli, Joni Augusto [UNESP] | |
dc.contributor.author | Deschner, James | |
dc.contributor.institution | Section of Experimental Dento-Maxillo-Facial Medicine | |
dc.contributor.institution | Johannes Gutenberg University | |
dc.contributor.institution | Center of Dento-Maxillo-Facial Medicine | |
dc.contributor.institution | Laboratory of Oral Microbiology | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2019-10-06T16:29:44Z | |
dc.date.available | 2019-10-06T16:29:44Z | |
dc.date.issued | 2019-04-25 | |
dc.description.abstract | Recently, it has been suggested that the anti-inflammatory hormone ghrelin (GHRL) and its receptor GHS-R may play a pivotal role in periodontal health and diseases. However, their exact regulation and effects in periodontitis are not known. The aim of this in-vitro study was to investigate the effect of microbial and inflammatory insults on the GHS-R1a expression in human osteoblast-like cells. MG-63 cells were exposed to interleukin (IL)-1β and Fusobacterium nucleatum in the presence and absence of GHRL for up to 2 d. Subsequently, gene expressions of GHS-R1a, inflammatory mediators and matrix metalloproteinase were analyzed by real-time PCR. GHS-R protein synthesis and NF-κB p65 nuclear translocation were assessed by immunocytochemistry and immunofluorescence microscopy, respectively. IL-1β and F. nucleatum caused a significant upregulation of GHS-R1a expression and an increase in GHS-R1a protein. Pre-incubation with a MEK1/2 inhibitor diminished the IL-1β-induced GHS-R1a upregulation. IL-1β and F. nucleatum also enhanced the expressions of cyclooxygenase 2, CC-chemokine ligand 2, IL-6, IL-8, and matrix metalloproteinase 1, but these stimulatory effects were counteracted by GHRL. By contrast, the stimulatory actions of IL-1β and F. nucleatum on the GHS-R1a expression were further enhanced by GHRL. Our study provides original evidence that IL-1β and F. nucleatum regulate the GHS-R/GHRL system in osteoblast-like cells. Furthermore, we demonstrate for the first time that the proinflammatory and proteolytic actions of IL-1β and F. nucleatum on osteoblast-like cells are inhibited by GHRL. Our study suggests that microbial and inflammatory insults upregulate GHS-R1a, which may represent a protective negative feedback mechanism in human bone. | en |
dc.description.affiliation | University of Bonn Center of Dento-Maxillo-Facial Medicine Section of Experimental Dento-Maxillo-Facial Medicine | |
dc.description.affiliation | University Medical Center Johannes Gutenberg University Department of Periodontology and Operative Dentistry | |
dc.description.affiliation | University of Bonn Center of Dento-Maxillo-Facial Medicine Department of Orthodontics | |
dc.description.affiliation | University of Bern Department of Periodontology Laboratory of Oral Microbiology | |
dc.description.affiliation | Universidade Estadual Paulista - UNESP School of Dentistry of Araraquara Department of Diagnosis and Surgery | |
dc.description.affiliationUnesp | Universidade Estadual Paulista - UNESP School of Dentistry of Araraquara Department of Diagnosis and Surgery | |
dc.format.extent | e025 | |
dc.identifier | http://dx.doi.org/10.1590/1807-3107bor-2019.vol33.0025 | |
dc.identifier.citation | Brazilian oral research, v. 33, p. e025-. | |
dc.identifier.doi | 10.1590/1807-3107bor-2019.vol33.0025 | |
dc.identifier.file | S1806-83242019000100221.pdf | |
dc.identifier.issn | 1807-3107 | |
dc.identifier.scielo | S1806-83242019000100221 | |
dc.identifier.scopus | 2-s2.0-85065481659 | |
dc.identifier.uri | http://hdl.handle.net/11449/189100 | |
dc.language.iso | eng | |
dc.relation.ispartof | Brazilian oral research | |
dc.rights.accessRights | Acesso aberto | pt |
dc.source | Scopus | |
dc.title | Regulation of ghrelin receptor by microbial and inflammatory signals in human osteoblasts | en |
dc.type | Artigo | pt |
dspace.entity.type | Publication | |
relation.isOrgUnitOfPublication | ca4c0298-cd82-48ee-a9c8-c97704bac2b0 | |
relation.isOrgUnitOfPublication.latestForDiscovery | ca4c0298-cd82-48ee-a9c8-c97704bac2b0 | |
unesp.author.orcid | 0000-0003-0998-1717[1] | |
unesp.author.orcid | 0000-0002-2756-5947[2] | |
unesp.author.orcid | 0000-0002-8153-6610[3] | |
unesp.author.orcid | 0000-0002-2706-398X[4] | |
unesp.author.orcid | 0000-0002-4619-2461[5] | |
unesp.author.orcid | 0000-0002-7082-9290[6] | |
unesp.author.orcid | 0000-0002-8808-8769[7] | |
unesp.campus | Universidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquara | pt |
unesp.department | Diagnóstico e Cirurgia - FOAR | pt |
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