Induction of mitotic crossing-over in diploid strains of Aspergillus nidulans using low-dose X-rays

As a contribution towards detecting the genetic effects of low doses of genotoxic physical agents, this paper deals with the consequences of low-dose X-rays in the Aspergillus nidulans genome. The irradiation doses studied were those commonly used in dental clinics (1-5 cGy). Even very low doses promoted increased mitotic crossing-over frequencies in diploid strains heterozygous for several genetic markers including the ones involved in DNA repair and recombination mechanisms. Genetic markers of several heterozygous strains were individually analyzed disclosing that some markers were especially sensitive to the treatments. These markers should be chosen as bio-indicators in the homozygotization index assay to better detect the recombinogenic/ carcinogenic genomic effects of low-dose X-rays. ©FUNPEC-RP.

Keywords

Aspergillus nidulans, DNA damage, Genotoxicity tests, Homozygotization index assay, Low-dose X-rays, Mitotic crossing-over, controlled study, crossing over, diploidy, DNA recombination, DNA repair, fungal gene, fungal strain, genotoxicity, low energy radiation, mitosis, nonhuman, radiation mutagenesis, X irradiation, Crossing Over, Genetic, Diploidy, DNA Damage, Dose-Response Relationship, Radiation, Homozygote, Mitosis, Mutagenicity Tests, X-Rays, Emericella nidulans