Publicação: Upregulation of mRNA myocardium calcium handling in rats submitted to exercise and food restriction
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Background: Chronic exercise and food restriction (FR) have directionally opposite changes in transcription of molecular structures of calcium handling and thyroid hormone (TH) status. Objective: Evaluate the association of chronic exercise and FR on serum thyroid hormones and gene transcription of molecular structures of intracellular calcium transients and thyroid receptors in myocardium of rats. Methods: Male Wistar Kyoto rats, divided into two groups: control (C, n = 7), FR (R50', n = 7), chronic exercise (EX, n = 7) and chronic exercise + FR (EX50', n = 7). FR was of 50% and exercise was swimming (1 hour/day, 5 days/ week, during 12 weeks). Serum concentrations of T3', T4 and TSH were determined. The mRNA gene expression of the sarcoplasmatic reticulum calcium pump (SERCA2a), phospholamban (PLB), Na+/Ca+2 exchanger (NCX), calcium channel L-type (L-channel), ryanodine (RYR), calsequestrin (CQS) and HT receptor (TRα1 and TRβ1) of the myocardium was performed by PCR real-time. Results: FR reduced serum levels of T4 and TSH and TRα1 mRNA and increased the expression of PLB, NCX and L-channel. Exercise increased the TRβ1 receptor, L-channel and NCX. The association of exercise and FR reduced plasma T4 and TSH, TRβ1 mRNA increase, SERCA2a, NCX and PLB, and there was a significant correlation of TRβ1 with CQS and NXC. Conclusion: Chronic exercise and food restriction increased the mRNA of transient Ca2+ proteins; however, TH - receptor axis cannot participate in the transcription of mRNA of myocardial calcium transient proteins.
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Calcium, Caloric restriction, Carrier proteins, Exercise, Myocardium, Thyroid hormones
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Inglês
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Arquivos Brasileiros de Cardiologia, v. 97, n. 1, 2011.
