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Fsh Stimulates Spermatogonial Proliferation and Differentiation in Zebrafish via Igf3

dc.contributor.authorNobrega, Rafael Henrique [UNESP]
dc.contributor.authorVidal de Souza Morais, Roberto Daltro
dc.contributor.authorCrespo, Diego
dc.contributor.authorWaal, Paul P. de
dc.contributor.authorFranca, Luiz Renato de
dc.contributor.authorSchulz, Rudiger W.
dc.contributor.authorBogerd, Jan
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Utrecht
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.date.accessioned2018-11-26T16:17:06Z
dc.date.available2018-11-26T16:17:06Z
dc.date.issued2015-10-01
dc.description.abstractGrowth factors modulate germ line stem cell self-renewal and differentiation behavior. We investigate the effects of Igf3, a fish-specific member of the igf family. Fsh increased in a steroid-independent manner the number and mitotic index of single type A undifferentiated spermatogonia and of clones of type A differentiating spermatogonia in adult zebrafish testis. All 4 igf gene family members in zebrafish are expressed in the testis but in tissue culture only igf3 transcript levels increased in response to recombinant zebrafish Fsh. This occurred in a cAMP/protein kinase A-dependent manner, in line with the results of studies on the igf3 gene promoter. Igf3 protein was detected in Sertoli cells. Recombinant zebrafish Igf3 increased the mitotic index of type A undifferentiated and type A differentiating spermatogonia and up-regulated the expression of genes related to spermatogonial differentiation and entry into meiosis, but Igf3 did not modulate testicular androgen release. An Igf receptor inhibitor blocked these effects of Igf3. Importantly, the Igf receptor inhibitor also blocked Fsh-induced spermatogonial proliferation. We conclude that Fsh stimulated Sertoli cell production of Igf3, which promoted via Igf receptor signaling spermatogonial proliferation and differentiation and their entry into meiosis. Because previous work showed that Fsh also released spermatogonia from an inhibitory signal by down-regulating anti Mullerian hormone and by stimulating androgen production, we can now present a model, in which Fsh orchestrates the activity of stimulatory (Igf3, androgens) and inhibitory (anti-Mullerian hormone) signals to promote spermatogenesis.en
dc.description.affiliationSao Paulo State Univ, Inst Biosci, Dept Morphol, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUniv Utrecht, Fac Sci, Dept Biol, Div Dev Biol,Reprod Biol Grp, NL-3584 CH Utrecht, Netherlands
dc.description.affiliationUniv Fed Minas Gerais, Inst Biol Sci, Dept Morphol, Lab Cellular Biol, BR-31270901 Belo Horizonte, MG, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Inst Biosci, Dept Morphol, BR-18618970 Botucatu, SP, Brazil
dc.description.sponsorshipUtrecht University
dc.description.sponsorshipEuropean Union
dc.description.sponsorshipNorwegian Research Council
dc.description.sponsorshipBrazilian funding agency Coordination for the Improvement of Higher Level Personnel
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipBrazilian funding agency National Council for Scientific and Technological Development
dc.description.sponsorshipIdEuropean Union: LIFECYCLE FP7-222719
dc.description.sponsorshipIdNorwegian Research Council: 159045
dc.format.extent3804-3817
dc.identifierhttp://dx.doi.org/10.1210/en.2015-1157
dc.identifier.citationEndocrinology. Washington: Endocrine Soc, v. 156, n. 10, p. 3804-3817, 2015.
dc.identifier.doi10.1210/en.2015-1157
dc.identifier.fileWOS000362235300044.pdf
dc.identifier.issn0013-7227
dc.identifier.lattes0515708585253985
dc.identifier.orcid0000-0001-9796-5076
dc.identifier.urihttp://hdl.handle.net/11449/160877
dc.identifier.wosWOS:000362235300044
dc.language.isoeng
dc.publisherEndocrine Soc
dc.relation.ispartofEndocrinology
dc.relation.ispartofsjr1,878
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleFsh Stimulates Spermatogonial Proliferation and Differentiation in Zebrafish via Igf3en
dc.typeArtigo
dcterms.rightsHolderEndocrine Soc
dspace.entity.typePublication
unesp.author.lattes0515708585253985[1]
unesp.author.orcid0000-0001-9796-5076[1]
unesp.author.orcid0000-0001-8477-3390[3]
unesp.author.orcid0000-0003-1120-7974[7]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatupt
unesp.departmentMorfologia - IBBpt

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