Fsh Stimulates Spermatogonial Proliferation and Differentiation in Zebrafish via Igf3
dc.contributor.author | Nobrega, Rafael Henrique [UNESP] | |
dc.contributor.author | Vidal de Souza Morais, Roberto Daltro | |
dc.contributor.author | Crespo, Diego | |
dc.contributor.author | Waal, Paul P. de | |
dc.contributor.author | Franca, Luiz Renato de | |
dc.contributor.author | Schulz, Rudiger W. | |
dc.contributor.author | Bogerd, Jan | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Univ Utrecht | |
dc.contributor.institution | Universidade Federal de Minas Gerais (UFMG) | |
dc.date.accessioned | 2018-11-26T16:17:06Z | |
dc.date.available | 2018-11-26T16:17:06Z | |
dc.date.issued | 2015-10-01 | |
dc.description.abstract | Growth factors modulate germ line stem cell self-renewal and differentiation behavior. We investigate the effects of Igf3, a fish-specific member of the igf family. Fsh increased in a steroid-independent manner the number and mitotic index of single type A undifferentiated spermatogonia and of clones of type A differentiating spermatogonia in adult zebrafish testis. All 4 igf gene family members in zebrafish are expressed in the testis but in tissue culture only igf3 transcript levels increased in response to recombinant zebrafish Fsh. This occurred in a cAMP/protein kinase A-dependent manner, in line with the results of studies on the igf3 gene promoter. Igf3 protein was detected in Sertoli cells. Recombinant zebrafish Igf3 increased the mitotic index of type A undifferentiated and type A differentiating spermatogonia and up-regulated the expression of genes related to spermatogonial differentiation and entry into meiosis, but Igf3 did not modulate testicular androgen release. An Igf receptor inhibitor blocked these effects of Igf3. Importantly, the Igf receptor inhibitor also blocked Fsh-induced spermatogonial proliferation. We conclude that Fsh stimulated Sertoli cell production of Igf3, which promoted via Igf receptor signaling spermatogonial proliferation and differentiation and their entry into meiosis. Because previous work showed that Fsh also released spermatogonia from an inhibitory signal by down-regulating anti Mullerian hormone and by stimulating androgen production, we can now present a model, in which Fsh orchestrates the activity of stimulatory (Igf3, androgens) and inhibitory (anti-Mullerian hormone) signals to promote spermatogenesis. | en |
dc.description.affiliation | Sao Paulo State Univ, Inst Biosci, Dept Morphol, BR-18618970 Botucatu, SP, Brazil | |
dc.description.affiliation | Univ Utrecht, Fac Sci, Dept Biol, Div Dev Biol,Reprod Biol Grp, NL-3584 CH Utrecht, Netherlands | |
dc.description.affiliation | Univ Fed Minas Gerais, Inst Biol Sci, Dept Morphol, Lab Cellular Biol, BR-31270901 Belo Horizonte, MG, Brazil | |
dc.description.affiliationUnesp | Sao Paulo State Univ, Inst Biosci, Dept Morphol, BR-18618970 Botucatu, SP, Brazil | |
dc.description.sponsorship | Utrecht University | |
dc.description.sponsorship | European Union | |
dc.description.sponsorship | Norwegian Research Council | |
dc.description.sponsorship | Brazilian funding agency Coordination for the Improvement of Higher Level Personnel | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorship | Brazilian funding agency National Council for Scientific and Technological Development | |
dc.description.sponsorshipId | European Union: LIFECYCLE FP7-222719 | |
dc.description.sponsorshipId | Norwegian Research Council: 159045 | |
dc.format.extent | 3804-3817 | |
dc.identifier | http://dx.doi.org/10.1210/en.2015-1157 | |
dc.identifier.citation | Endocrinology. Washington: Endocrine Soc, v. 156, n. 10, p. 3804-3817, 2015. | |
dc.identifier.doi | 10.1210/en.2015-1157 | |
dc.identifier.file | WOS000362235300044.pdf | |
dc.identifier.issn | 0013-7227 | |
dc.identifier.lattes | 0515708585253985 | |
dc.identifier.orcid | 0000-0001-9796-5076 | |
dc.identifier.uri | http://hdl.handle.net/11449/160877 | |
dc.identifier.wos | WOS:000362235300044 | |
dc.language.iso | eng | |
dc.publisher | Endocrine Soc | |
dc.relation.ispartof | Endocrinology | |
dc.relation.ispartofsjr | 1,878 | |
dc.rights.accessRights | Acesso aberto | |
dc.source | Web of Science | |
dc.title | Fsh Stimulates Spermatogonial Proliferation and Differentiation in Zebrafish via Igf3 | en |
dc.type | Artigo | |
dcterms.rightsHolder | Endocrine Soc | |
dspace.entity.type | Publication | |
unesp.author.lattes | 0515708585253985[1] | |
unesp.author.orcid | 0000-0001-9796-5076[1] | |
unesp.author.orcid | 0000-0001-8477-3390[3] | |
unesp.author.orcid | 0000-0003-1120-7974[7] | |
unesp.campus | Universidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatu | pt |
unesp.department | Morfologia - IBB | pt |
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