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Evaluation of early changes induced by diuron in the rat urinary bladder using different processing methods for scanning electron microscopy

dc.contributor.authorFava, Rafaela Marono [UNESP]
dc.contributor.authorFerragut Cardoso, Ana Paula [UNESP]
dc.contributor.authorRocha, Mitscheli Sanches da
dc.contributor.authorNascimento e Pontes, Merielen Garcia [UNESP]
dc.contributor.authorViana de Camargo, Joao Lauro [UNESP]
dc.contributor.authorCotrim Sartor de Oliveira, Maria Luiza [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionPlanitox
dc.date.accessioned2015-10-21T13:09:27Z
dc.date.available2015-10-21T13:09:27Z
dc.date.issued2015-07-03
dc.description.abstractDiuron [3-(3,4-dichlorophenyl)-1,1-dimethylurea] is a substituted urea herbicide carcinogenic to the rat urinary bladder at high dietary levels. The suggested non-genotoxic mode of action (MOA) of diuron encompasses cytotoxicity and necrosis followed by regenerative hyperplasia. Prenecrotic swollen cells as observed under scanning electron microscopy (SEM) have been reported as early morphological alterations, putatively related to diuron cytotoxicity. However, these changes were not observed in a previous SEM study conducted in this laboratory. This study evaluated whether these early alterations are actually due to diuron cytotoxicity or artifacts related to different processing methods used for SEM analysis. Male Wistar rats were fed ad libitum with basal diet, 7.1% sodium saccharin (NaS) or 2.500 ppm diuron for seven days or 15 weeks. The urinary bladders were processed for histological and labeling indices examinations and for SEM using two different processing methods. The incidence of simple hyperplasia after 15 weeks of exposure to diuron or to NaS was significantly increased. By SEM, the incidences and severity of lesions were significantly increased in the diuron group independently of exposure time. The different SEM processing methods used allowed for visualization of swollen superficial cells after seven days of diuron exposure. Probably the absence these cells in a previous study was due to the use very few animals. Our results support the hypothesis that the swollen cell is an early key event due to diuron-induced cytotoxicity and is the result of a degenerative process involved in the non-genotoxic carcinogenic mode of action of high doses of diuron.en
dc.description.affiliationSão Paulo State University (UNESP) - Center for the Evaluation of the Environmental Impact on Human Health (TOXICAM), Department of Pathology, Botucatu Medical School, Botucatu, São Paulo 18618-970, Brazil
dc.description.affiliationPlanitox, Campinas, 13024-230 SP, Brazil
dc.description.affiliationUnespSão Paulo State University (UNESP) - Center for the Evaluation of the Environmental Impact on Human Health (TOXICAM), Department of Pathology, Botucatu Medical School, Botucatu, São Paulo 18618-970, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipCenter for the Evaluation of the Environmental Impact on Human Health (TOXICAM), Department of Pathology, Sao Paulo State University (UNESP) Medical School, Botucatu, SP, Brazil
dc.description.sponsorshipIdFAPESP: 2012/11210-3
dc.format.extent100-106
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0300483X15000773
dc.identifier.citationToxicology. Clare: Elsevier Ireland Ltd, v. 333, p. 100-106, 2015.
dc.identifier.doi10.1016/j.tox.2015.04.006
dc.identifier.issn0300-483X
dc.identifier.urihttp://hdl.handle.net/11449/128385
dc.identifier.wosWOS:000357228200010
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofToxicology
dc.relation.ispartofjcr3.265
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectDiuron[3-(3,4-dichlorophenyl)-1,1-dimethylurea]en
dc.subjectCarcinogenesisen
dc.subjectEarly changeen
dc.subjectRat urinary bladderen
dc.subjectScanning electron microscopyen
dc.subjectSwollen cellsen
dc.titleEvaluation of early changes induced by diuron in the rat urinary bladder using different processing methods for scanning electron microscopyen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina, Botucatupt
unesp.departmentPatologia - FMBpt

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