Nanoskin extracellular matrix modified with hyaluronic acid and chondroitin sulfate for regenerative medicine applications

Abstract

Natural extracellular matrices (ECMs) perform the tasks necessary for tissue formation, maintenance, regulation and function, providing a powerful means of controlling the biological performance of regenerative materials. The extracellular matrix represents Mother Nature's biologic scaffold. The ECM is rich in structural and functional proteins that not only maintain the health of differentiated tissues, but support the host response to tissue injury. In this work, we present the extracellular matrix (ECM) using the bacterial cellulose (Nanoskin®) which regulates cell behavior by influencing cell proliferation, survival, shape, migration and differentiation. Bacterial cellulose fermentation process is modified by the addition of vegetal stem cell to the culture medium and natural materials (hyaluronic acid and chondroitin sulfate) before the bacteria are inoculated for mimicking ECM to cells support and built new skin formation.