Publicação: How can micelle systems be rebuilt by a heating process?
dc.contributor.author | da Silva-Filho, Miguel Adelino | |
dc.contributor.author | Vieira da Silva Siqueira, Scheyla Daniela | |
dc.contributor.author | Freire, Larissa Bandeira | |
dc.contributor.author | de Araujo, Ivonete Batista | |
dc.contributor.author | Gyselle de Holanda e Silva, Kattya | |
dc.contributor.author | Medeiros, Aldo da Cunha | |
dc.contributor.author | Araujo-Filho, Irami | |
dc.contributor.author | Oliveira, Anselmo Gomes de [UNESP] | |
dc.contributor.author | Tabosa do Egito, Eryvaldo Socrates | |
dc.contributor.institution | Universidade Federal do Rio Grande do Norte (UFRN) | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2014-05-20T13:25:02Z | |
dc.date.available | 2014-05-20T13:25:02Z | |
dc.date.issued | 2012-01-01 | |
dc.description.abstract | The aim of this work was to evaluate how an aqueous micellar system - containing Amphotericin B (AmB) and sodium deoxycholate (DOC) can be rebuilt after heating treatment. Also, a review of the literature on the physicochemical and biological properties of this new system was conducted. Heated (AmB-DOC-H) and unheated (AmB-DOC) micelles were then diluted at four different concentrations (50 mg.L-1, 5 mg.L-1, 0.5 mg.L-1, and 0.05 mg.L-1) to perform physicochemical studies and a pharmacotoxicity assay, in which two cell models were used for the in vitro experiments: red blood cells (RBC) from human donors and Candida parapsilosis (Cp). While potassium (K+) and hemoglobin leakage from RBC were the parameters used to evaluate acute and chronic toxicity, respectively, the efficacy of AmB-DOC and AmB-DOC-H were assessed by K+ leakage and cell survival rate from Cp. The spectral study revealed a slight change in the AmB-DOC aggregate peak from 327 nm to 323 nm, which is the peak for AmB-DOC-H. Although AmB-DOC and AmB-DOC-H exhibited different behavior for hemoglobin leakage, AmB-DOC produced higher leakage than AmB-DOC-H at high concentrations (from 5 mg.L-1). For K+ leakage, both AmB-DOC and AmB-DOC-H showed a similar profile for both cell models, RBC and Cp (P < 0.05). AmB-DOC-H and AmB-DOC also revealed a similar profile of activity against Cp with an equivalent survival rate. In short, AmB-DOC-H showed much less toxicity than AmB-DOC, but remained as active as AmB-DOC against fungal cells. The results highlight the importance of this new procedure as a simple, inexpensive, and safe way to produce a new kind of micelle system for the treatment of systemic fungal infections. | en |
dc.description.affiliation | Fed Univ Rio Grande Norte UFRN, Dispersed Syst Lab, BR-59094450 Natal, RN, Brazil | |
dc.description.affiliation | Univ Fed Rio Grande do Norte, Dept Pharm, Natal, RN, Brazil | |
dc.description.affiliation | Univ Fed Rio Grande do Norte, Dept Expt Surg, Natal, RN, Brazil | |
dc.description.affiliation | Coll Pharmaceut Sci UNESP, Dept Drugs & Med, São Paulo, Brazil | |
dc.description.affiliationUnesp | Coll Pharmaceut Sci UNESP, Dept Drugs & Med, São Paulo, Brazil | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description.sponsorshipId | CNPq: 301979/04-9 | |
dc.description.sponsorshipId | CNPq: 473882/04-3 | |
dc.description.sponsorshipId | CNPq: 47836/01-7-NV | |
dc.format.extent | 141-150 | |
dc.identifier | http://dx.doi.org/10.2147/IJN.S25761 | |
dc.identifier.citation | International Journal of Nanomedicine. Albany: Dove Medical Press Ltd, v. 7, p. 141-150, 2012. | |
dc.identifier.doi | 10.2147/IJN.S25761 | |
dc.identifier.file | WOS000302700700001.pdf | |
dc.identifier.issn | 1178-2013 | |
dc.identifier.lattes | 9114495952533044 | |
dc.identifier.uri | http://hdl.handle.net/11449/7920 | |
dc.identifier.wos | WOS:000302700700001 | |
dc.language.iso | eng | |
dc.publisher | Dove Medical Press Ltd | |
dc.relation.ispartof | International Journal of Nanomedicine | |
dc.relation.ispartofjcr | 4.370 | |
dc.relation.ispartofsjr | 1,225 | |
dc.rights.accessRights | Acesso aberto | pt |
dc.source | Web of Science | |
dc.subject | micelles | en |
dc.subject | nanotechnology | en |
dc.subject | pre-heated amphotericin B | en |
dc.subject | super-aggregates | en |
dc.title | How can micelle systems be rebuilt by a heating process? | en |
dc.type | Artigo | pt |
dcterms.license | http://www.dovepress.com/why_publish_with_dove.php?content_id=3045 | |
dcterms.rightsHolder | Dove Medical Press Ltd | |
dspace.entity.type | Publication | |
relation.isDepartmentOfPublication | e214da1b-9929-4ae9-b8fd-655e9bfeda4b | |
relation.isDepartmentOfPublication.latestForDiscovery | e214da1b-9929-4ae9-b8fd-655e9bfeda4b | |
relation.isOrgUnitOfPublication | 95697b0b-8977-4af6-88d5-c29c80b5ee92 | |
relation.isOrgUnitOfPublication.latestForDiscovery | 95697b0b-8977-4af6-88d5-c29c80b5ee92 | |
unesp.author.lattes | 9114495952533044[8] | |
unesp.author.orcid | 0000-0002-2180-3991[9] | |
unesp.author.orcid | 0000-0002-0107-9940[8] | |
unesp.author.orcid | 0000-0002-9997-4870[1] | |
unesp.author.orcid | 0000-0003-2471-7447[7] | |
unesp.campus | Universidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquara | pt |
unesp.department | Fármacos e Medicamentos - FCF | pt |
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