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REDUCTION OF CELL PROLIFERATIVE ACTIVITIES OF GASTRIC STUMP ADENOMATOUS HYPERPLASIAS AFTER BILE REFLUX DIVERSION IN RATS

dc.contributor.authorImai, T.
dc.contributor.authorKobayasi, Shoiti [UNESP]
dc.contributor.authorRodrigues, MAM
dc.contributor.authorDecamargo, JLV
dc.contributor.authorOgawa, K.
dc.contributor.authorIwata, H.
dc.contributor.authorTatematsu, M.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionNAGOYA CITY UNIV
dc.date.accessioned2014-05-20T15:29:44Z
dc.date.available2014-05-20T15:29:44Z
dc.date.issued1993-09-01
dc.description.abstractPreviously we reported the majority of lesions induced hy bile reflux, in the absence of chemical carcinogens, in the rat remnant stomach to consist primarily of gastric type and secondarily of intestinal type cells, and that they are reversible after diversion of bile reflux. The present study was designed to evaluate changes in proliferative activities in cells of each type under these conditions. The frequency of adenomatous hyperplasia (AH) induced in the gastric stump mucosa by duodenal content reflux after Billroth II partial gastrectomy (BII) increased until the 54th week of the experiment. Roux-en-Y (RY) surgical procedure which prevents duodenal reflux performed at the 24th or 36th week after BII led to a decrease in AH. Cell content of the lesions was analyzed using routine H&E staining, immunohistochemical staining for pepsinogen isoenzyme 1 and histochemical procedures for mucins (paradoxical concanavalin A, galactose oxidase Schiff and sialidase galactose oxidase Schiff reactions) and proliferation in each compartment evaluated by an immunohistochemical method using bromodeoxyuridine (BrdU) and a monoclonal antibody against BrdU. At the 54th week the number of BrdU-labeled cells per normal pyloric column was significantly (P < 0.05) increased to 10.63/pit after the BII operation, while it diminished to 5.23/pit after RY diversion, this being the same level as with the RY procedure alone. AH maintained a high rate of BrdU incorporation at 12.7% after BII operation, which was also significantly reduced (P < 0.01) to 7.0% by the RY surgery. The intestinal type cell showed highest (22.2%), the surface mucous type cell showed the next (16.5%) and the pyloric gland type cell showed lowest (5.2%) BrdU labeling indices after BII operation. All the cell types in AH showed similar proportional decreases in BrdU incorporation after RY diversion. Thus surgical intervention reverses the cell proliferation caused by bile reflux in the gastric stump.en
dc.description.affiliationUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,FAC MED,DEPT CIRURGIA,BR-18600 BOTUCATU,SP,BRAZIL
dc.description.affiliationUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,FAC MED,DEPT PATOL,BR-18600 BOTUCATU,SP,BRAZIL
dc.description.affiliationNAGOYA CITY UNIV,SCH MED,DEPT PATHOL 1,NAGOYA,AICHI 467,JAPAN
dc.description.affiliationUnespUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,FAC MED,DEPT CIRURGIA,BR-18600 BOTUCATU,SP,BRAZIL
dc.description.affiliationUnespUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,FAC MED,DEPT PATOL,BR-18600 BOTUCATU,SP,BRAZIL
dc.format.extent1765-1769
dc.identifierhttp://dx.doi.org/10.1093/carcin/14.9.1765
dc.identifier.citationCarcinogenesis. Oxford: Oxford Univ Press United Kingdom, v. 14, n. 9, p. 1765-1769, 1993.
dc.identifier.doi10.1093/carcin/14.9.1765
dc.identifier.issn0143-3334
dc.identifier.lattes3191019476459702
dc.identifier.urihttp://hdl.handle.net/11449/39246
dc.identifier.wosWOS:A1993LY80400007
dc.language.isoeng
dc.publisherOxford University Press
dc.relation.ispartofCarcinogenesis
dc.relation.ispartofjcr5.072
dc.relation.ispartofsjr2,135
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleREDUCTION OF CELL PROLIFERATIVE ACTIVITIES OF GASTRIC STUMP ADENOMATOUS HYPERPLASIAS AFTER BILE REFLUX DIVERSION IN RATSen
dc.typeArtigo
dcterms.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dcterms.rightsHolderOxford Univ Press United Kingdom
dspace.entity.typePublication
unesp.author.lattes3191019476459702
unesp.author.orcid0000-0003-3833-4172[4]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina, Botucatupt
unesp.departmentPatologia - FMBpt

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