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Cytotoxicity of Portland Cement with Different Radiopacifying Agents: A Cell Death Study

dc.contributor.authorGomes Cornelio, Ana Livia [UNESP]
dc.contributor.authorSalles, Loise Pedrosa [UNESP]
dc.contributor.authorda Paz, Mariana Campos
dc.contributor.authorCirelli, Joni Augusto [UNESP]
dc.contributor.authorGuerreiro-Tanomaru, Juliane Maria [UNESP]
dc.contributor.authorTanomaru Filho, Mario [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de Brasília (UnB)
dc.date.accessioned2013-09-30T18:31:52Z
dc.date.accessioned2014-05-20T13:45:26Z
dc.date.available2013-09-30T18:31:52Z
dc.date.available2014-05-20T13:45:26Z
dc.date.issued2011-02-01
dc.description.abstractIntroduction: The aim of this study was to investigate the cytotoxicity of white Portland cement (PC) alone or associated with bismuth oxide (PCBi), zirconium oxide (PCZir), and calcium tungstate (PCCa) in 2 cell lineages. Methods: Murine periodontal ligament cells (mPDL) and rat osteosarcoma cells (ROS 17/2.8) were exposed for 24 hours to specific concentrations of fresh PC and PC associations with radiopacifiers. Zinc oxide-eugenol cement and hydrogen peroxide treatment were applied as cytotoxic positive controls. Cell viability after incubation with the cements was assessed by mitochondrial dehydrogenase enzymatic assay. Cell morphology was microscopically analyzed by cresyl violet staining, and the mechanism of cell death was determined by acridine orange/ethidium bromide methodology. All data were analyzed statistically by analysis of variance and Tukey post hoc test (P < .05). The correlation among cell death by apoptosis or necrosis and pH values was established by Pearson linear coefficient. Results: The mitochondrial dehydrogenase enzymatic assay only revealed significant cell death rate at high concentrations of cement elutes. PC alone was not cytotoxic, even at 100 mg/ml. Microscopic images showed that none of the PC formulations caused damage to any cell lines. Statistical analysis of apoptosis/necrosis data demonstrated that PC and PC plus radiopacifying agents promoted significant necrosis cell death only at 100 mg/mL. Conclusions: The mPDL cells were more sensitive than ROS17/2.8. The results showed that PC associated with bismuth oxide, zirconium oxide, or calcium tungstate is not cytotoxic to mPDL or ROS17/2.8. Zirconium oxide and calcium tungstate might be good alternatives as radiopacifying agents. (J Endod 2011;37:203-210)en
dc.description.affiliationSão Paulo State Univ, UNESP, Dept Restorat Dent, Araraquara Dent Sch, Araraquara, SP, Brazil
dc.description.affiliationUniversidade de Brasilia (UnB), Dept Cellular & Mol Biol, Inst Biol Sci, BR-70910900 Brasilia, DF, Brazil
dc.description.affiliationUniversidade de Brasilia (UnB), Dept Genet & Morphol, Inst Biol Sci, BR-70910900 Brasilia, DF, Brazil
dc.description.affiliationSão Paulo State Univ, UNESP, Dept Diag & Surg, Araraquara Dent Sch, Araraquara, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Dept Restorat Dent, Araraquara Dent Sch, Araraquara, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Dept Diag & Surg, Araraquara Dent Sch, Araraquara, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent203-210
dc.identifierhttp://dx.doi.org/10.1016/j.joen.2010.11.017
dc.identifier.citationJournal of Endodontics. New York: Elsevier B.V., v. 37, n. 2, p. 203-210, 2011.
dc.identifier.doi10.1016/j.joen.2010.11.017
dc.identifier.issn0099-2399
dc.identifier.lattes2628593693450121
dc.identifier.lattes2665211298584751
dc.identifier.urihttp://hdl.handle.net/11449/15981
dc.identifier.wosWOS:000287428200014
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Endodontics
dc.relation.ispartofjcr2.886
dc.relation.ispartofsjr1,585
dc.rights.accessRightsAcesso restritopt
dc.sourceWeb of Science
dc.subjectApoptosisen
dc.subjectcytotoxicityen
dc.subjectPortland cementen
dc.subjectradiopacifying agenten
dc.titleCytotoxicity of Portland Cement with Different Radiopacifying Agents: A Cell Death Studyen
dc.typeArtigopt
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dspace.entity.typePublication
relation.isDepartmentOfPublication901124bf-5736-4432-b057-7c29dec84b50
relation.isDepartmentOfPublication.latestForDiscovery901124bf-5736-4432-b057-7c29dec84b50
relation.isOrgUnitOfPublicationca4c0298-cd82-48ee-a9c8-c97704bac2b0
relation.isOrgUnitOfPublication.latestForDiscoveryca4c0298-cd82-48ee-a9c8-c97704bac2b0
unesp.author.lattes2628593693450121
unesp.author.lattes2665211298584751
unesp.author.orcid0000-0002-2574-4706[6]
unesp.author.orcid0000-0002-7082-9290[4]
unesp.author.orcid0000-0003-0446-2037[5]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquarapt
unesp.departmentDiagnóstico e Cirurgia - FOARpt
unesp.departmentOdontologia Restauradora - FOARpt

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