Publicação: Acquired pellicle engineering with proteins/peptides: Mechanism of action on native human enamel surface
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Elsevier B.V.
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Objective: This study investigated the mechanism of action of different proteins/peptides (separately or in combination), focusing on how they act directly on the native enamel surface and on modifying the salivary pellicle. Methods: A total of 170 native human enamel specimens were prepared and submitted to different treatments (2 h; 37 degrees C): with deionized water, CaneCPI-5, Hemoglobin, Statherin, or a combination of all three proteins/peptides. The groups were subdivided into treatment acting on the enamel surface (NoP - absence of salivary pellicle), and treatment modifying the salivary pellicle (P). Treatment was made (2 h; 37 degrees C) in all specimens, and later, for P, the specimens were incubated in human saliva (2 h; 37 degrees C). In both cases, the specimens were immersed in 1% citric acid (pH 3.6; 2 min; 25 degrees C). Calcium released from enamel (CaR) and its relative surface reflection intensity (%SRI) was measured after 5 cycles. Between-group differences were verified with two-way ANOVA, with presence of pellicle and treatment as factors (alpha = 0.05). Results: The presence of pellicle provided better protection regarding %SRI (p < 0.01), but not regarding CaR (p = 0.201). In relation to treatment, when compared to the control group, all proteins/peptides provided significantly better protection (p < 0.01 for %SRI and Car). The combination of all three proteins/peptides demonstrated the best protective effect (p < 0.01 for %SRI). Conclusion: Depending on the protein or peptide, its erosion-inhibiting effect derives from their interaction with the enamel surface or from modifying the pellicle, so a combination of proteins and peptides provides the best protection. Clinical significance: The present study opens a new direction for a possible treatment with a combination of proteins for native human enamel, which can act directly on the enamel surface as well on the modification of the salivary pellicle, for the prevention of dental erosion.
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Acquired pellicle, Dental erosion, Enamel, Protein, Pellicle modification, saliva
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Inglês
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Journal Of Dentistry. Oxford: Elsevier Sci Ltd, v. 107, 7 p., 2021.
