Publicação: Evaluation of pH effects on genomic integrity in adipose-derived mesenchymal stem cells using the comet assay
Carregando...
Data
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Funpec-editora
Tipo
Artigo
Direito de acesso
Acesso restrito
Resumo
The use of mesenchymal stem cells (MSCs) in experimental, clinical, and therapeutic trials has grown in recent years. However, the issue remains of whether these procedures are completely safe for transplant patients. Therefore, this study was designed and carried out with the aim of evaluating two different comet assay protocols for genomic damage pattern analysis in MSCs derived from adipose tissue. The analyzed and interpreted results suggest that genetic testing is needed to support clonal expansion safety in cell therapy procedures with MSCs. Furthermore, they also suggest that if the comet assay technique would be used as a genomic integrity screening assay, the protocol performed at pH = 12 (that yielded a frequency of damaged cells: tail intensity = 9.50 +/- 0.60, tail moment = 0.0122 +/- 0.0007; results are reported as means +/- standard deviation) would be indicated as genomic damage, and that subsequent single-strand breaks occur at pH > 13 (frequency of damaged cells: tail intensity = 30.71 +/- 4.23, tail moment = 0.0447 +/- 0.0073). Our study demonstrates that, in the era of regenerative medicine, it is necessary to standardize and establish a battery of tests in order to identify genomic damage prior to MSC transplantation.
Descrição
Palavras-chave
Mesenchymal stem cells, Comet assay, Adipose tissue
Idioma
Inglês
Como citar
Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 14, n. 1, p. 339-348, 2015.
