Publicação:
Genetic Instability Persists in Non-Neoplastic Urothelial Cells from Patients with a History of Urothelial Cell Carcinoma

dc.contributor.authorCastro Marcondes, Joao Paulo de [UNESP]
dc.contributor.authorOliveira, Maria Luiza Cotrim Sartor de [UNESP]
dc.contributor.authorGontijo, Alisson M.
dc.contributor.authorCamargo, João Lauro Viana de [UNESP]
dc.contributor.authorSalvadori, Daisy Maria Favero [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Nova Lisboa
dc.date.accessioned2014-12-03T13:07:02Z
dc.date.available2014-12-03T13:07:02Z
dc.date.issued2014-01-22
dc.description.abstractBladder cancer is one of the most common genitourinary neoplasms in industrialized countries. Multifocality and high recurrence rates are prominent clinical features of this disease and contribute to its high morbidity. Therefore, more sensitive and less invasive techniques could help identify individuals with asymptomatic disease. In this context, we used the micronucleus assay to evaluate whether cytogenetic alterations could be used as biomarkers for monitoring patients with a history of urothelial cell carcinoma (UCC). We determined the frequency of micronucleated urothelial cells (MNC) in exfoliated bladder cells from 105 patients with (n = 52) or without (n = 53) a history of UCC, all of whom tested negative for neoplasia by cytopathological and histopathological analyses. MNC frequencies were increased in patients with a history of UCC (non-smoker and smoker/ex-smoker patients vs non-smoker and smoker/ex-smoker controls; p<0.001), in non-smoker UCC patients (vs non-smoker controls; p<0.01), and in smoker/ex-smoker controls (vs non-smoker controls; p<0.001). Patients with a history of recurrent disease also demonstrated a higher MNC frequency compared to patients with non-recurrent neoplasia. However, logistic regression using smoking habits, age and gender as confounding factors did not confirm MNC frequency as a marker for UCC recurrence. Fluorescent in situ hybridization analysis (using a pan-centromeric probe) showed that micronuclei (MN) arose mainly from clastogenic events regardless of UCC and/or smoking histories. In conclusion, our results confirm previous indications that subjects with a history of UCC harbor genetically unstable cells in the bladder urothelium. Furthermore, these results support using the micronucleus assay as an important tool for monitoring patients with a history of UCC and tumor recurrence.en
dc.description.affiliationUNESP Univ Estadual Paulista, Fac Med, Botucatu, SP, Brazil
dc.description.affiliationUniv Nova Lisboa, Fac Ciencias Med FCM, Ctr Estudos Doencas Cronicas, P-1200 Lisbon, Portugal
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Fac Med, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 05/55594-6
dc.description.sponsorshipIdCNPq: 301079/2009-9
dc.format.extent6
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0086162
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 9, n. 1, 6 p., 2014.
dc.identifier.doi10.1371/journal.pone.0086162
dc.identifier.fileWOS000330283100118.pdf
dc.identifier.issn1932-6203
dc.identifier.lattes5051118752980903
dc.identifier.urihttp://hdl.handle.net/11449/111191
dc.identifier.wosWOS:000330283100118
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPLOS ONE
dc.relation.ispartofjcr2.766
dc.relation.ispartofsjr1,164
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleGenetic Instability Persists in Non-Neoplastic Urothelial Cells from Patients with a History of Urothelial Cell Carcinomaen
dc.typeArtigo
dcterms.rightsHolderPublic Library Science
dspace.entity.typePublication
unesp.author.lattes5051118752980903
unesp.author.orcid0000-0001-9323-3134[5]
unesp.author.orcid0000-0002-2530-3708[3]
unesp.author.orcid0000-0002-5116-2494[1]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina, Botucatupt
unesp.departmentPatologia - FMBpt

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