Publicação: Direct detection of infectious bursal disease virus from clinical samples by in situ reverse transcriptase-linked polymerase chain reaction
dc.contributor.author | Cardoso, Tereza C. [UNESP] | |
dc.contributor.author | Rosa, Ana C. G. | |
dc.contributor.author | Astolphi, Rafael D. [UNESP] | |
dc.contributor.author | Vincente, Rafael M. [UNESP] | |
dc.contributor.author | Novais, Juliana B. [UNESP] | |
dc.contributor.author | Hirata, Karina Y. [UNESP] | |
dc.contributor.author | Luvizotto, Maria Cecilia R. [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2013-09-30T18:31:14Z | |
dc.date.accessioned | 2014-05-20T13:44:41Z | |
dc.date.available | 2013-09-30T18:31:14Z | |
dc.date.available | 2014-05-20T13:44:41Z | |
dc.date.issued | 2008-01-01 | |
dc.description.abstract | The presence of the very virulent (vv) Brazilian strain of infectious bursal disease virus (IBDV) was determined in the bursa of Fabricius, thymus and liver of 2-week-old broilers from a flock with a higher than expected mortality. For this purpose, a direct in situ reverse transcriptase (RT)-linked polymerase chain reaction (PCR) method was developed using specific primers for vvIBDV. Unlabelled forward and reverse biotinylated oligonucleotides were used for RT-PCR in a one-step method and the respective products were revealed by a direct enzymatic reaction. The results were compared with those obtained by standard RT-PCR using general primers for IBDV and virus isolation. The virus isolation, RT-PCR and in situ RT-PCR revealed positive results on the bursa of Fabricius in 86%, 80% and 100%, respectively. The in situ RT-PCR detected vvIBDV in all tested thymus and liver samples, whereas the standard RT-PCR detected virus in 80% and 90% of the samples, respectively. After three consecutive passages on chicken embryonated eggs, IBDV was isolated from 64% of the thymus samples and 30% of the liver samples. In the present study, no classical or antigenic variants of IBDV were detected. The developed in situ RT-PCR assay was able to detect the very virulent strain of IBDV with a higher sensitivity than the conventional RT-PCR and virus isolation. | en |
dc.description.affiliation | Univ Estadual Paulista, Virol Lab, DAPSA, São Paulo, Brazil | |
dc.description.affiliation | Univ Estadual Paulista, Curso Med Vet, DCCRA, Lab Patol, BR-16050680 Aracatuba, SP, Brazil | |
dc.description.affiliationUnesp | Univ Estadual Paulista, Virol Lab, DAPSA, São Paulo, Brazil | |
dc.description.affiliationUnesp | Univ Estadual Paulista, Curso Med Vet, DCCRA, Lab Patol, BR-16050680 Aracatuba, SP, Brazil | |
dc.format.extent | 457-461 | |
dc.identifier | http://dx.doi.org/10.1080/03079450802216587 | |
dc.identifier.citation | Avian Pathology. Abingdon: Taylor & Francis Ltd, v. 37, n. 4, p. 457-461, 2008. | |
dc.identifier.doi | 10.1080/03079450802216587 | |
dc.identifier.issn | 0307-9457 | |
dc.identifier.uri | http://hdl.handle.net/11449/15664 | |
dc.identifier.wos | WOS:000257592700016 | |
dc.language.iso | eng | |
dc.publisher | Taylor & Francis Ltd | |
dc.relation.ispartof | Avian Pathology | |
dc.relation.ispartofjcr | 2.054 | |
dc.relation.ispartofsjr | 0,871 | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Web of Science | |
dc.title | Direct detection of infectious bursal disease virus from clinical samples by in situ reverse transcriptase-linked polymerase chain reaction | en |
dc.type | Artigo | |
dcterms.license | http://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp | |
dcterms.rightsHolder | Taylor & Francis Ltd | |
dspace.entity.type | Publication | |
unesp.campus | Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatuba | pt |
unesp.department | Clínica, Cirurgia e Reprodução Animal - FMVA | pt |
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