Logo do repositório
 

Identification of quantitative trait loci affecting resistance to gastrointestinal parasites in a double backcross population of Red Maasai and Dorper sheep

dc.contributor.authorSilva, M. V. B.
dc.contributor.authorSonstegard, T. S.
dc.contributor.authorHanotte, O.
dc.contributor.authorMugambi, J. M.
dc.contributor.authorGarcia, J. F.
dc.contributor.authorNagda, S.
dc.contributor.authorGibson, J. P.
dc.contributor.authorIraqi, F. A.
dc.contributor.authorMcClintock, A. E.
dc.contributor.authorKemp, S. J.
dc.contributor.authorBoettcher, P. J.
dc.contributor.authorMalek, M.
dc.contributor.authorVan Tassell, C. P.
dc.contributor.authorBaker, R. L.
dc.contributor.institutionARS
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionInt Livestock Res Inst
dc.contributor.institutionUniv Nottingham
dc.contributor.institutionVet Res Ctr KARI
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFAO IAEA Agr & Biotechnol Lab
dc.contributor.institutionHawkins Homestead Univ New England Armidale
dc.contributor.institutionTel Aviv Univ
dc.date.accessioned2014-05-20T15:31:55Z
dc.date.available2014-05-20T15:31:55Z
dc.date.issued2012-02-01
dc.description.abstractA genome-wide scan for quantitative trait loci (QTL) affecting gastrointestinal nematode resistance in sheep was completed using a double backcross population derived from Red Maasai and Dorper ewes bred to F1 rams. This design provided an opportunity to map potentially unique genetic variation associated with a parasite-tolerant breed like Red Maasai, a breed developed to survive East African grazing conditions. Parasite indicator phenotypes (blood packed cell volume PCV and faecal egg count FEC) were collected on a weekly basis from 1064 lambs during a single 3-month post-weaning grazing challenge on infected pastures. The averages of last measurements for FEC (AVFEC) and PCV (AVPCV), along with decline in PCV from challenge start to end (PCVD), were used to select lambs (N = 371) for genotyping that represented the tails (10% threshold) of the phenotypic distributions. Marker genotypes for 172 microsatellite loci covering 25 of 26 autosomes (1560.7 cm) were scored and corrected by Genoprob prior to qxpak analysis that included BoxCox transformed AVFEC and arcsine transformed PCV statistics. Significant QTL for AVFEC and AVPCV were detected on four chromosomes, and this included a novel AVFEC QTL on chromosome 6 that would have remained undetected without BoxCox transformation methods. The most significant P-values for AVFEC, AVPCV and PCVD overlapped the same marker interval on chromosome 22, suggesting the potential for a single causative mutation, which remains unknown. In all cases, the favourable QTL allele was always contributed from Red Maasai, providing support for the idea that future marker-assisted selection for genetic improvement of production in East Africa will rely on markers in linkage disequilibrium with these QTL.en
dc.description.affiliationARS, USDA, Bovine Funct Genom Lab, Beltsville, MD 20705 USA
dc.description.affiliationEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Dairy Cattle, BR-36038330 Juiz de Fora, MG, Brazil
dc.description.affiliationInt Livestock Res Inst, Nairobi, Kenya
dc.description.affiliationUniv Nottingham, Sch Biol, Nottingham NG7 2RD, England
dc.description.affiliationVet Res Ctr KARI, Kikuyu 0092, Kenya
dc.description.affiliationUniv Estadual Paulista UNESP, BR-16050680 Aracatuba, SP, Brazil
dc.description.affiliationFAO IAEA Agr & Biotechnol Lab, A-1400 Vienna, Austria
dc.description.affiliationHawkins Homestead Univ New England Armidale, Inst Genet & Bioinformat, Armidale, NSW 2351, Australia
dc.description.affiliationTel Aviv Univ, Dept Human Microbiol, IL-69978 Tel Aviv, Israel
dc.description.affiliationUnespUniv Estadual Paulista UNESP, BR-16050680 Aracatuba, SP, Brazil
dc.description.sponsorshipUSAID
dc.description.sponsorshipUSDA Agricultural Research Service
dc.description.sponsorshipIdUSDA ARS: 1265-31000-084D (BFGL)
dc.format.extent63-71
dc.identifierhttp://dx.doi.org/10.1111/j.1365-2052.2011.02202.x
dc.identifier.citationAnimal Genetics. Hoboken: Wiley-blackwell, v. 43, n. 1, p. 63-71, 2012.
dc.identifier.doi10.1111/j.1365-2052.2011.02202.x
dc.identifier.issn0268-9146
dc.identifier.urihttp://hdl.handle.net/11449/40937
dc.identifier.wosWOS:000298874000009
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofAnimal Genetics
dc.relation.ispartofjcr1.841
dc.relation.ispartofsjr0,830
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectEast Africaen
dc.subjectgastrointestinal parasitesen
dc.subjectmarker-assisted selectionen
dc.subjectquantitative trait locien
dc.subjectsheepen
dc.titleIdentification of quantitative trait loci affecting resistance to gastrointestinal parasites in a double backcross population of Red Maasai and Dorper sheepen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell
dspace.entity.typePublication

Arquivos

Licença do pacote

Agora exibindo 1 - 2 de 2
Carregando...
Imagem de Miniatura
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Carregando...
Imagem de Miniatura
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções