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Impact of proximal cytoplasmic droplets on quality traits and in-vitro embryo production efficiency of cryopreserved bull spermatozoa

dc.contributor.authorCarreira, Janaina T. [UNESP]
dc.contributor.authorMingoti, Gisele Zoccal [UNESP]
dc.contributor.authorRodrigues, Lucia H.
dc.contributor.authorSilva, Carlos
dc.contributor.authorPerri, Silvia Helena Venturoli [UNESP]
dc.contributor.authorKoivisto, Marion B. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionCRVLagoa
dc.date.accessioned2013-09-30T18:25:10Z
dc.date.accessioned2014-05-20T13:42:35Z
dc.date.available2013-09-30T18:25:10Z
dc.date.available2014-05-20T13:42:35Z
dc.date.issued2012-01-12
dc.description.abstractBackground: Proximal cytoplasmic droplets (PCDs), a remnant of germ cell cytoplasm, are common non-specific morphological defects in bovine semen. This study evaluated the effect of higher percentages of PCDs on the quality of frozen-thawed bovine semen, embryo production and early embryo development.Methods: Three ejaculates from each of five (group 1: PCD <= 1%, control) and eight adult Bos indicus bulls (group 2: PCD >= 24%) were analysed. Semen samples were examined for: post-thaw motility, vigour of movement, concentration, sperm morphology, slow thermoresistance test (STT), membrane integrity, acrosome status, mitochondrial function using fluorescent probes association (FITC-PSA, PI and JC-1) and sperm chromatin integrity using acridine orange assay. Two bulls from group 2, with 28.5% and 48.5% PCD, respectively, and three bulls from the control group, each with 0% PCD, were selected for IVF (in vitro fertilisation).Results: Semen analyses revealed a significant correlation (P < 0.01) between increased rates of PCD and sperm quality traits. Nevertheless, no differences were observed in sperm motility and vigour either before or after the STT or in the percentage of intact acrosomes (analysed by differential interference contrast microscopy (DIC) after STT), but membrane integrity, acrosome status (evaluated with FITC-PSA staining method after thawing) and mitochondrial function were reduced, when compared with group 1 (P < 0.05). The higher incidence of PCD was positively correlated to chromatin damage, especially after three hours of incubation at 37 degrees C. IVF showed similar results for bull C2 (group 1, control) and bull P2 (group 2, group with higher PCDs).Conclusion: Higher PCD levels influenced spermatozoa quality traits. IVF and embryo development data showed that cleavage, blastocyst formation and blastocyst hatching may have been influenced by the interaction of morphology traits and individual bull effects.en
dc.description.affiliationUniv Estadual Paulista UNESP, FMVA, Fac Vet Med, Dept Clin Surg & Anim Reprod, Aracatuba, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, Dept Support Prod & Anim Hlth, Aracatuba, Brazil
dc.description.affiliationCRVLagoa, Sertaozinho, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, FMVA, Fac Vet Med, Dept Clin Surg & Anim Reprod, Aracatuba, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Dept Support Prod & Anim Hlth, Aracatuba, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 06/03537-1
dc.format.extent7
dc.identifierhttp://dx.doi.org/10.1186/1751-0147-54-1
dc.identifier.citationActa Veterinaria Scandinavica. London: Biomed Central Ltd., v. 54, p. 7, 2012.
dc.identifier.doi10.1186/1751-0147-54-1
dc.identifier.fileWOS000300973900001.pdf
dc.identifier.issn0044-605X
dc.identifier.lattes4191951764334766
dc.identifier.lattes0810837598202634
dc.identifier.orcid0000-0002-3059-4458
dc.identifier.urihttp://hdl.handle.net/11449/14808
dc.identifier.wosWOS:000300973900001
dc.language.isoeng
dc.publisherBiomed Central Ltd.
dc.relation.ispartofActa Veterinaria Scandinavica
dc.relation.ispartofjcr1.497
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectsperm morphologyen
dc.subjectproximal cytoplasmic dropletsen
dc.subjectIVFen
dc.subjectfluorescent probesen
dc.titleImpact of proximal cytoplasmic droplets on quality traits and in-vitro embryo production efficiency of cryopreserved bull spermatozoaen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
dcterms.rightsHolderBiomed Central Ltd.
dspace.entity.typePublication
unesp.author.lattes4191951764334766
unesp.author.lattes0810837598202634
unesp.author.orcid0000-0002-3059-4458[2]
unesp.author.orcid0000-0001-5083-5557[5]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatubapt
unesp.departmentClínica, Cirurgia e Reprodução Animal - FMVApt
unesp.departmentApoio, Produção e Saúde Animal - FMVApt

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