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Effect of Using Two Cryopreservation Methods on Viability and Fertility of Frozen Stallion Sperm

dc.contributor.authorMaziero, Rosiara Rosaria Dias
dc.contributor.authorGuaitolini, Carlos Renato de Freitas [UNESP]
dc.contributor.authorGuasti, Priscilla Nascimento [UNESP]
dc.contributor.authorMonteiro, Gabriel Augusto
dc.contributor.authorMartin, Ian
dc.contributor.authorSilva, Juliano Pianowski Marques da
dc.contributor.authorCrespilho, André Maciel
dc.contributor.authorPapa, Frederico Ozanam [UNESP]
dc.contributor.institutionParananense University
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionMinas Gerais Federal University
dc.contributor.institutionUberaba University
dc.contributor.institutionSanto Amaro University
dc.date.accessioned2019-10-06T16:05:23Z
dc.date.available2019-10-06T16:05:23Z
dc.date.issued2019-01-01
dc.description.abstractStudies involving different methods and techniques of cryopreservation and its interactions with the conception rates in artificial insemination (AI) programs are reported in the literature. This study evaluated the sperm kinetics, plasma membrane integrity, and fertility rates of mares inseminated with cryopreserved stallion semen subjected to different freezing methods. For this, four ejaculates from five stallions were collected and frozen in conventional (Styrofoam box) or automated system in Mini-Digitcool ZH 400. Seminal samples were evaluated after thawing for sperm motion parameters by CASA and plasma membrane integrity by epifluorescence microscopy. For the fertility trial, a cross-over model was performed using 100 cycles of 50 mares, which were inseminated by one the two freezing methods. No differences were observed for sperm motion parameters and plasma membrane integrity between groups (P >.05). The pregnancy rate using the conventional method was 56% (28/50) and did not differ (P =.5406) from the pregnancy rate (64%, 32/50) obtained using the automatized method. The use of semen from fertile stallions may not illustrate small differences in the two freezing methods evaluated. Conventional and automated freezing systems did not differ in the quality and viability of fertile stallion semen and conception rates, indicating that the two methodologies can be safely used in AI programs.en
dc.description.affiliationDepartment of Animal Reproduction Parananense University
dc.description.affiliationDepartment of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science UNESP
dc.description.affiliationDepartment of Animal Reproduction Minas Gerais Federal University
dc.description.affiliationDepartment of Animal Science Uberaba University
dc.description.affiliationDepartment of Animal Science Santo Amaro University
dc.description.affiliationUnespDepartment of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science UNESP
dc.format.extent37-40
dc.identifierhttp://dx.doi.org/10.1016/j.jevs.2018.10.008
dc.identifier.citationJournal of Equine Veterinary Science, v. 72, p. 37-40.
dc.identifier.doi10.1016/j.jevs.2018.10.008
dc.identifier.issn0737-0806
dc.identifier.scopus2-s2.0-85056470614
dc.identifier.urihttp://hdl.handle.net/11449/188354
dc.language.isoeng
dc.relation.ispartofJournal of Equine Veterinary Science
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectEquine
dc.subjectFertility
dc.subjectFreezing protocol
dc.subjectFreezing rate
dc.subjectSemen cryopreservation
dc.titleEffect of Using Two Cryopreservation Methods on Viability and Fertility of Frozen Stallion Spermen
dc.typeArtigo
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.departmentReprodução Animal e Radiologia Veterinária - FMVZpt

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