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Biostimulatory effects of low-level laser therapy on epithelial cells and gingival fibroblasts treated with zoledronic acid

dc.contributor.authorBasso, F. G.
dc.contributor.authorPansani, T. N. [UNESP]
dc.contributor.authorTurrioni, A. P S [UNESP]
dc.contributor.authorKurachi, C.
dc.contributor.authorBagnato, V. S.
dc.contributor.authorHebling, J. [UNESP]
dc.contributor.authorde Souza Costa, C. A. [UNESP]
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:29:02Z
dc.date.available2014-05-27T11:29:02Z
dc.date.issued2013-05-01
dc.description.abstractLow-level laser therapy (LLLT) has been considered as an adjuvant treatment for bisphosphonate-related osteonecrosis, presenting positive clinical outcomes. However, there are no data regarding the effect of LLLT on oral tissue cells exposed to bisphosphonates. This study aimed to evaluate the effects of LLLT on epithelial cells and gingival fibroblasts exposed to a nitrogen-containing bisphosphonate - zoledronic acid (ZA). Cells were seeded in wells of 24-well plates, incubated for 48 h and then exposed to ZA at 5 μM for an additional 48 h. LLLT was performed with a diode laser prototype - LaserTABLE (InGaAsP - 780 nm ± 3 nm, 25 mW), at selected energy doses of 0.5, 1.5, 3, 5, and 7 J cm-2 in three irradiation sessions, every 24 h. Cell metabolism, total protein production, gene expression of vascular endothelial growth factor (VEGF) and collagen type I (Col-I), and cell morphology were evaluated 24 h after the last irradiation. Data were statistically analyzed by Kruskal-Wallis and Mann-Whitney tests at 5% significance. Selected LLLT parameters increased the functions of epithelial cells and gingival fibroblasts treated with ZA. Gene expression of VEGF and Col-I was also increased. Specific parameters of LLLT biostimulated fibroblasts and epithelial cells treated with ZA. Analysis of these in vitro data may explain the positive in vivo effects of LLLT applied to osteonecrosis lesions. © 2013 Astro Ltd.en
dc.description.affiliationUNICAMP - Universidade de Campinas Piracicaba School of Dentistry, SP, 13414-903, Piracicaba
dc.description.affiliationUNESP - Universidade Estadual Paulista Araraquara School of Dentistry, SP, 14801-903, Araraquara
dc.description.affiliationUSP - Universidade de São Paulo Physics Institute, São Carlos, SP
dc.description.affiliationUnespUNESP - Universidade Estadual Paulista Araraquara School of Dentistry, SP, 14801-903, Araraquara
dc.identifierhttp://dx.doi.org/10.1088/1054-660X/23/5/055601
dc.identifier.citationLaser Physics, v. 23, n. 5, 2013.
dc.identifier.doi10.1088/1054-660X/23/5/055601
dc.identifier.issn1054-660X
dc.identifier.issn1555-6611
dc.identifier.scopus2-s2.0-84879122110
dc.identifier.urihttp://hdl.handle.net/11449/75247
dc.identifier.wosWOS:000318007600025
dc.language.isoeng
dc.relation.ispartofLaser Physics
dc.relation.ispartofjcr1.158
dc.relation.ispartofsjr0,345
dc.rights.accessRightsAcesso restritopt
dc.sourceScopus
dc.subjectAdjuvant treatment
dc.subjectCell metabolism
dc.subjectClinical outcome
dc.subjectEpithelial cells
dc.subjectGingival fibroblasts
dc.subjectLow level laser therapy
dc.subjectVascular endothelial growth factor
dc.subjectZoledronic acid
dc.subjectFibroblasts
dc.subjectGene expression
dc.subjectIrradiation
dc.subjectLaser tissue interaction
dc.subjectTissue
dc.subjectCell culture
dc.titleBiostimulatory effects of low-level laser therapy on epithelial cells and gingival fibroblasts treated with zoledronic aciden
dc.typeArtigopt
dcterms.licensehttp://iopscience.iop.org/page/copyright
dspace.entity.typePublication
relation.isOrgUnitOfPublicationca4c0298-cd82-48ee-a9c8-c97704bac2b0
relation.isOrgUnitOfPublication.latestForDiscoveryca4c0298-cd82-48ee-a9c8-c97704bac2b0
unesp.author.lattes4517484241515548[7]
unesp.author.orcid0000-0002-5931-6849[2]
unesp.author.orcid0000-0002-7455-6867[7]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquarapt

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