Logotipo do repositório
 

Publicação:
Testicular cytology by fine needle aspiration in domestic cats

dc.contributor.authorLeme, D. P.
dc.contributor.authorVisacre, E.
dc.contributor.authorCastro, V. B.
dc.contributor.authorLopes, M. D. [UNESP]
dc.contributor.institutionUniversidade Federal de Santa Catarina (UFSC)
dc.contributor.institutionVeterinary Hospital-UNIP
dc.contributor.institutionFederal University of Recôncavo da Bahia
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:15:21Z
dc.date.available2018-12-11T17:15:21Z
dc.date.issued2018-01-15
dc.description.abstractIn cases where semen collection in tom-cats is not possible, FNA of testes is the alternative to evaluate sperm production. Although this technique for the diagnosis of fertility problems has been well discussed in other mammals (men, dogs, stallions), data for domestic cats are limited. Therefore, the aim of this study was to verify the reliability of FNA using needles of small diameters (22G and 29G) in testes of domestic cats of different ages to assess the spermatogenesis status and to present description of germ cells and Sertoli cells for cytological examinations. Thirty-four mixed breed cats aged between four months and two years presented for neutering to a Veterinary Hospital were used in this study. Under general anesthesia, testicular measures and FNA were followed by orchiectomy and imprints of the parenchyma of testes and epididymides. Cats were assigned into 3 groups: (G1) 10 cats aged less than 6 months, (G2) 14 cats aged between 6 months and one year and (G3) 10 cats aged more than one year. Cats weighted between 1.5 and 6.0 kg. The mean testicular volume (TV cm3) was 0.55 (G1), 1.18 (G2) and 2.66 (G3). Hemorrhages in the needle path were observed in more than 70% of testes. Few samples (4/68) were excluded due to blood contamination. All germ cells and Sertoli cells were identified and quantified in imprint and FNA smears. Incomplete spermatogenesis was observed in cats aged less than 6 months using both techniques (FNA and imprint); therefore, testicular FNA should not be recommended for cats at this age. Complete spermatogenesis was found in 64% of cats aged from 6 months up to one year and in all cats aged more than one year. There were no differences of Sertoli cell Index (SEI) and Spermatic Index (SI) between FNA and imprints of cats older than 6 months. In conclusion, FNA using needles of small diameter in the testes of domestic cats is viable, reliable and can be used as a tool for the analysis of the spermatogenesis status of cats older than 6 months, mainly in cases in which semen collection is not possible.en
dc.description.affiliationDepartment of Rural Development and Animal Sciences LETA CCA Federal University of Santa Catarina
dc.description.affiliationVeterinary Hospital-UNIP
dc.description.affiliationCenter for Agrarian Environmental and Biological Sciences Federal University of Recôncavo da Bahia
dc.description.affiliationDepartment of Animal Reproduction and Veterinary Radiology FMVZ UNESP
dc.description.affiliationUnespDepartment of Animal Reproduction and Veterinary Radiology FMVZ UNESP
dc.format.extent46-52
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2017.10.012
dc.identifier.citationTheriogenology, v. 106, p. 46-52.
dc.identifier.doi10.1016/j.theriogenology.2017.10.012
dc.identifier.file2-s2.0-85031298439.pdf
dc.identifier.issn0093-691X
dc.identifier.scopus2-s2.0-85031298439
dc.identifier.urihttp://hdl.handle.net/11449/175337
dc.language.isoeng
dc.relation.ispartofTheriogenology
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectFeline
dc.subjectFNA
dc.subjectImprint
dc.subjectSpermatogenesis
dc.subjectTestes
dc.titleTesticular cytology by fine needle aspiration in domestic catsen
dc.typeArtigo
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.departmentReprodução Animal e Radiologia Veterinária - FMVZpt

Arquivos

Pacote Original

Agora exibindo 1 - 1 de 1
Carregando...
Imagem de Miniatura
Nome:
2-s2.0-85031298439.pdf
Tamanho:
1.03 MB
Formato:
Adobe Portable Document Format
Descrição: