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Are NORs Always Located on Homeologous Chromosomes? A FISH Investigation with rDNA and Whole Chromosome Probes in Gymnotus Fishes (Gymnotiformes)

dc.contributor.authorMilhomem, Susana S. R.
dc.contributor.authorScacchetti, Priscilla C. [UNESP]
dc.contributor.authorPieczarka, Julio C.
dc.contributor.authorFerguson-Smith, Malcolm A.
dc.contributor.authorPansonato-Alves, José C. [UNESP]
dc.contributor.authorO'Brien, Patricia C. M.
dc.contributor.authorForesti, Fausto [UNESP]
dc.contributor.authorNagamachi, Cleusa Y.
dc.contributor.institutionUniversidade Federal do Pará (UFPA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Cambridge
dc.date.accessioned2014-05-27T11:28:21Z
dc.date.available2014-05-27T11:28:21Z
dc.date.issued2013-02-06
dc.description.abstractGymnotus (Gymnotiformes, Gymnotidae) is the most diverse known Neotropical electric knife fish genus. Cytogenetic studies in Gymnotus demonstrate a huge karyotypic diversity for this genus, with diploid numbers ranging from 34 to 54. The NOR are also variable in this genus, with both single and multiple NORs described. A common interpretation is that the single NOR pair is a primitive trait while multiple NORs are derivative. However this hypothesis has never been fully tested. In this report we checked if the NOR-bearing chromosome and the rDNA site are homeologous in different species of the genus Gymnotus: G. carapo (2n = 40, 42, 54), G. mamiraua (2n = 54), G. arapaima (2n = 44), G. sylvius (2n = 40), G. inaequilabiatus (2n = 54) and G. capanema (2n = 34), from the monophyletic group G. carapo (Gymnotidae-Gymnotiformes), as well as G. jonasi (2n = 52), belonging to the G1 group. They were analyzed with Fluorescence in situ hybridization (FISH) using 18S rDNA and whole chromosome probes of the NOR-bearing chromosome 20 (GCA20) of G. carapo (cytotype 2n = 42), obtained by Fluorescence Activated Cell Sorting. All species of the monophyletic G. carapo group show the NOR in the same single pair, confirmed by hybridization with CGA20 whole chromosome probe. In G. jonasi the NORs are multiple, and located on pairs 9, 10 and 11. In G. jonasi the GCA20 chromosome probe paints the distal half of the long arm of pair 7, which is not a NOR-bearing chromosome. Thus these rDNA sequences are not always in the homeologous chromosomes in different species thus giving no support to the hypothesis that single NOR pairs are primitive traits while multiple NORs are derived. The separation of groups of species in the genus Gymnotus proposed by phylogenies with morphologic and molecular data is supported by our cytogenetic data. © 2013 Milhomem et al.en
dc.description.affiliationLaboratório de Citogenética Instituto de Ciências Biológicas Universidade Federal do Pará, Belém, Pará
dc.description.affiliationInstituto de Biociências de Botucatu Departamento de Morfologia Universidade Estadual Paulista, Botucatu, São Paulo
dc.description.affiliationCambridge Resource Centre for Comparative Genomics Department of Veterinary Medicine University of Cambridge, Cambridge
dc.description.affiliationUnespInstituto de Biociências de Botucatu Departamento de Morfologia Universidade Estadual Paulista, Botucatu, São Paulo
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0055608
dc.identifier.citationPLoS ONE, v. 8, n. 2, 2013.
dc.identifier.doi10.1371/journal.pone.0055608
dc.identifier.file2-s2.0-84873531614.pdf
dc.identifier.issn1932-6203
dc.identifier.lattes0804793944846367
dc.identifier.scopus2-s2.0-84873531614
dc.identifier.urihttp://hdl.handle.net/11449/74563
dc.identifier.wosWOS:000315153400123
dc.language.isoeng
dc.relation.ispartofPLOS ONE
dc.relation.ispartofjcr2.766
dc.relation.ispartofsjr1,164
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectribosome DNA
dc.subjectchromosomal localization
dc.subjectchromosome analysis
dc.subjectchromosome NOR
dc.subjectcontrolled study
dc.subjectcytotype
dc.subjectDNA determination
dc.subjectDNA sequence
dc.subjectfish
dc.subjectfluorescence activated cell sorting
dc.subjectfluorescence in situ hybridization
dc.subjectGymnotus arapaima
dc.subjectGymnotus capanema
dc.subjectGymnotus carapo
dc.subjectGymnotus inaequilabiatus
dc.subjectGymnotus jonasi
dc.subjectGymnotus mamiraua
dc.subjectGymnotus sylvius
dc.subjectmolecular probe
dc.subjectnonhuman
dc.subjectspecies difference
dc.subjectAnimals
dc.subjectChromosomes
dc.subjectDNA Probes
dc.subjectDNA, Ribosomal
dc.subjectGymnotiformes
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectNucleolus Organizer Region
dc.subjectPhylogeny
dc.subjectSpecies Specificity
dc.titleAre NORs Always Located on Homeologous Chromosomes? A FISH Investigation with rDNA and Whole Chromosome Probes in Gymnotus Fishes (Gymnotiformes)en
dc.typeArtigo
dcterms.licensehttp://www.plos.org/open-access/
dspace.entity.typePublication
unesp.author.lattes0804793944846367
unesp.author.orcid0000-0003-2951-8877[3]
unesp.author.orcid0000-0003-3580-7384[5]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatupt
unesp.departmentMorfologia - IBBpt

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