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Determination of carbamate pesticide in food using a biosensor based on reduced graphene oxide and acetylcholinesterase enzyme

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Abstract

Food safety is a major concern for human health and wellbeing all over the world. A novel and sensitive biosensor based on reduced graphene oxide (rGO) and the enzyme acetylcholinesterase (AChE) was developed and applied for the detection of carbaryl in food samples. The glassy carbon/rGO/AChE biosensor was characterized morphologically and electrochemically using scanning electron microscopy and cyclic voltammetry/electrochemical impedance spectroscopy, respectively. Optimum differential pulse voltammetry conditions led to a nanomolar detection limit, and determination of carbaryl in tomato was achieved.

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Acetylcholinesterase enzyme, Biosensor, Carbaryl, Food safety, Reduced graphene oxide

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English

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Sensors and Actuators, B: Chemical, v. 277, p. 555-561.

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