Publicação: An electrochemical aptasensor for detection of bovine interferon gamma
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2017-08-21
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Royal Soc Chemistry
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Bovine tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB) in which it is hard to identify the pathological symptoms. Release of bovine interferon gamma (BoIFN-gamma) by T-cells provides an important diagnostic marker of MTB infections. In this work, we developed for the first time an electrochemical aptasensor for sensitive and specific determination of BoIFN-gamma. A thiolated IFN-gbinding aptamer was conjugated with methylene blue (MB) and immobilized on a gold electrode by selfassembly. Binding of IFN-g to the electrode surface caused a conformational change in the aptamer, decreasing electron transfer efficiency. The redox current was quantified using square wave voltammetry (SWV) and was found to be specific for bovine IFN-g with detection limit of 0.1 nM in pristine buffer and 0.9 nM in blood. The biosensor described here may, in the future, be used for on-site testing of bovine blood to help better identify and contain outbreaks of bovine TB.
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Analytical Methods. Cambridge: Royal Soc Chemistry, v. 9, n. 31, p. 4527-4532, 2017.
