Logo do repositório
 

Subinhibitory Concentrations of Triclosan Promote Streptococcus mutans Biofilm Formation and Adherence to Oral Epithelial Cells

dc.contributor.authorLombardo Bedran, Telma Blanca [UNESP]
dc.contributor.authorGrignon, Louis
dc.contributor.authorSpolidorio, Denise Palomari [UNESP]
dc.contributor.authorGrenier, Daniel
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Laval
dc.date.accessioned2014-12-03T13:10:46Z
dc.date.available2014-12-03T13:10:46Z
dc.date.issued2014-02-13
dc.description.abstractTriclosan is a general membrane-active agent with a broad-spectrum antimicrobial activity that is commonly used in oral care products. In this study, we investigated the effect of sub-minimum inhibitory concentrations (MICs) of triclosan on the capacity of the cariogenic bacterium Streptococcus mutans to form biofilm and adhere to oral epithelial cells. As quantified by crystal violet staining, biofilm formation by two reference strains of S. mutans was dose-dependently promoted, in the range of 2.2- to 6.2-fold, by 1/2 and 1/4 MIC of triclosan. Observations by scanning electron microscopy revealed the presence of a dense biofilm attached to the polystyrene surface. Growth of S. mutans in the presence of triclosan at subMICs also increased its capacity to adhere to a monolayer of gingival epithelial cells. The expression of several genes involved in adherence and biofilm formation in S. mutans was investigated by quantitative RT-PCR. It was found that subMICs of triclosan significantly increased the expression of comD, gtfC, and luxS, and to a lesser extent of gtfB and atlA genes. These findings stress the importance of maintaining effective bactericidal concentrations of therapeutic triclosan since subMICs may promote colonization of the oral cavity by S. mutans.en
dc.description.affiliationState Univ Sao Paulo, Araraquara Dent Sch, Dept Oral Diag & Surg, Sao Paulo, Brazil
dc.description.affiliationUniv Laval, Fac Dent, Oral Ecol Res Grp, Quebec City, PQ, Canada
dc.description.affiliationState Univ Sao Paulo, Araraquara Dent Sch, Dept Physiol & Pathol, Sao Paulo, Brazil
dc.description.affiliationUnespState Univ Sao Paulo, Araraquara Dent Sch, Dept Oral Diag & Surg, Sao Paulo, Brazil
dc.description.affiliationUnespState Univ Sao Paulo, Araraquara Dent Sch, Dept Physiol & Pathol, Sao Paulo, Brazil
dc.description.sponsorshipCanadian Institutes of Health Research
dc.description.sponsorshipIdCanadian Institutes of Health Research95335
dc.format.extent6
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0089059
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 9, n. 2, 6 p., 2014.
dc.identifier.doi10.1371/journal.pone.0089059
dc.identifier.fileWOS000331266000097.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11449/112510
dc.identifier.wosWOS:000331266000097
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPLOS ONE
dc.relation.ispartofjcr2.766
dc.relation.ispartofsjr1,164
dc.rights.accessRightsAcesso abertopt
dc.sourceWeb of Science
dc.titleSubinhibitory Concentrations of Triclosan Promote Streptococcus mutans Biofilm Formation and Adherence to Oral Epithelial Cellsen
dc.typeArtigopt
dcterms.rightsHolderPublic Library Science
dspace.entity.typePublication
relation.isDepartmentOfPublicationb3ba3d9c-022e-4521-8805-0bcceea7372e
relation.isDepartmentOfPublication.latestForDiscoveryb3ba3d9c-022e-4521-8805-0bcceea7372e
relation.isOrgUnitOfPublicationca4c0298-cd82-48ee-a9c8-c97704bac2b0
relation.isOrgUnitOfPublication.latestForDiscoveryca4c0298-cd82-48ee-a9c8-c97704bac2b0
unesp.author.orcid0000-0003-2376-1024[3]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquarapt
unesp.departmentDiagnóstico e Cirurgia - FOARpt
unesp.departmentFisiologia e Patologia - FOARpt

Arquivos

Pacote original

Agora exibindo 1 - 1 de 1
Carregando...
Imagem de Miniatura
Nome:
WOS000331266000097.pdf
Tamanho:
5.38 MB
Formato:
Adobe Portable Document Format