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Histologic study of use of microfibrillar collagen hemostat in rat dental sockets

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Fundação Odontológica de Ribeirão Preto

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Abstract (portuguese)

O objetivo deste trabalho é avaliar se a colocação do MCH no interior do alvéolo dental interfere ou não no reparo alveolar. Foi feita anestesia geral nos trinta ratos machos adultos Albinus Wistar e o incisivo central superior direito foi extraído. No grupo controle, após a exodontia o alvéolo foi suturado. No grupo MCH, após a exodontia introduziu-se MCH dentro do alvéolo que foi suturado. No pós-operatório, cinco animais de cada grupo foram sacrificados aos 7, 21 e 28 dias. Retirou-se a maxila direita de cada animal e cortes histológicos foram confeccionados. Foi feita análise qualitativa e quantitativa. A porcentagem de área óssea no interior dos alvéolos dentais foi quantificada usando o sistema de análise de imagens ImageLab 98. A área óssea neoformada no grupo controle e no grupo MCH foi: 8.1% e 3.3% aos 7 dias, 34.4% e 33% aos 21 dias e 41% e 41.3% aos 28 dias, respectivamente. Dentro das condições experimentais deste trabalho foi possível concluir que o MCH interfere durante o início do reparo alveolar e não interfere no reparo final do alvéolo dental.

Abstract (english)

The aim of this paper was to evaluate if the placement of microfibrillar collagen hemostat (MCH) into a dental socket interfered with healing. General anesthesia was administered to 30 adult male Albinus Wistar rats and the maxillary right central incisor was extracted. In the control group after each tooth was extracted, the socket was sutured. In the MCH group after each tooth was extracted, MCH was placed into the socket before suturing. Postoperatively, 5 animals were sacrificed from each group at 7, 21 and 28 days. The right maxilla was removed from each animal and histologic slides were stained with Masson's trichromic and hematoxylin and eosin. Quantitative and qualitative analyses were done. The percentage of bone area in the dental socket was quantified using the Image Lab 98 image analysis system. The bone area formation for the control and MCH groups was: 8.1% and 3.3% at 7 days, 34.4% and 33% at 21 days and 41% and 41.3% at 28 days, respectively. We concluded that MCH interferes with the beginning of dental socket healing but does not interfere with the final healing of the dental socket.

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English

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Brazilian Dental Journal. Fundação Odontológica de Ribeirão Preto, v. 14, n. 1, p. 12-15, 2003.

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Faculdade de Odontologia
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Campus: Araçatuba

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