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Expression of Protease Activated Receptor-1 in Chronic Periodontitis

dc.contributor.authorBueno da Silva, Henrique Aparecido
dc.contributor.authorEuzebio Alves, Vanessa Tubero
dc.contributor.authorSpolidório, Luis Carlos [UNESP]
dc.contributor.authorCesar Neto, Joao Batista
dc.contributor.authorEichler, Rosangela Santos
dc.contributor.authorCatelli de Carvalho, Maria Helena
dc.contributor.authorHolzhausen, Marinella
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-03-18T15:56:11Z
dc.date.available2015-03-18T15:56:11Z
dc.date.issued2014-12-01
dc.description.abstractBackground: Protease activated receptor-1 (PAR(1)) activation by thrombin may play a role in repair and homeostasis of periodontal tissues. The main objective of this study is to investigate PAR(1) expression in patients with periodontitis, before and after non-surgical periodontal treatment, and to associate its expression with the presence of inflammatory biomarkers and PAR(2) expression.Methods: Gingival crevicular fluid (GCF) samples and clinical parameters, including probing depth, clinical attachment level, bleeding on probing, and gingival and plaque indices, were collected from periodontally healthy individuals and patients with moderate chronic periodontitis (CP) before and 6 weeks after periodontal non-surgical treatment. PAR(1) and PAR(2) messenger RNA (mRNA) at the GCF were evaluated by quantitative polymerase chain reaction (qPCR). Flow cytometry analysis identified the GCF PAR(1)-expressing cells. GCF inflammatory biomarkers were also determined.Results: Clinical parameters were significantly improved after therapy (P < 0.01). The qPCR analysis showed that, before therapy, PAR(1) mRNA levels in CP were similar to controls. Periodontal treatment led to increased PAR(1) expression in CP (P < 0.05). PAR(1) expression was inversely correlated to PAR(2) expression and with interleukins 6 and 8, tumor necrosis factor-alpha, interferon-gamma, and matrix metalloproteinase-2 levels.Conclusions: Periodontal treatment results in PAR(1) overexpression in the GCF, and PAR(1) expression is associated with decreased expression of inflammatory biomarkers and inversely correlated to PAR(2) expression in the GCF. Therefore, the data suggest the importance of PAR(1) mediating the known anabolic actions of thrombin in the periodontium.en
dc.description.affiliationUniv Sao Paulo, Sch Dent, Dept Stomatol, Div Periodont, BR-05508000 Sao Paulo, Brazil
dc.description.affiliationState Univ Sao Paulo, Dent Sch Araraquara, Dept Physiol & Pathol, Araraquara, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Inst Biomed Sci, Dept Pharmacol, BR-05508000 Sao Paulo, Brazil
dc.description.affiliationUnespState Univ Sao Paulo, Dent Sch Araraquara, Dept Physiol & Pathol, Araraquara, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordination for the Improvement of Upper Education Personnel
dc.description.sponsorshipNational Council of Scientific and Technological Development
dc.description.sponsorshipIdFAPESP: 10/16605-0
dc.format.extent1763-1769
dc.identifierhttp://dx.doi.org/10.1902/jop.2014.140172
dc.identifier.citationJournal Of Periodontology. Chicago: Amer Acad Periodontology, v. 85, n. 12, p. 1763-1769, 2014.
dc.identifier.doi10.1902/jop.2014.140172
dc.identifier.issn0022-3492
dc.identifier.lattes2640929291808415
dc.identifier.urihttp://hdl.handle.net/11449/117451
dc.identifier.wosWOS:000345744300017
dc.language.isoeng
dc.publisherAmer Acad Periodontology
dc.relation.ispartofJournal Of Periodontology
dc.relation.ispartofjcr3.392
dc.relation.ispartofsjr1,408
dc.rights.accessRightsAcesso restritopt
dc.sourceWeb of Science
dc.subjectBiological markersen
dc.subjectchronic periodontitisen
dc.subjectgene expressionen
dc.subjectimmunity, innateen
dc.subjectinflammationen
dc.subjectwound healingen
dc.titleExpression of Protease Activated Receptor-1 in Chronic Periodontitisen
dc.typeArtigopt
dcterms.rightsHolderAmer Acad Periodontology
dspace.entity.typePublication
relation.isDepartmentOfPublicationb3ba3d9c-022e-4521-8805-0bcceea7372e
relation.isDepartmentOfPublication.latestForDiscoveryb3ba3d9c-022e-4521-8805-0bcceea7372e
relation.isOrgUnitOfPublicationca4c0298-cd82-48ee-a9c8-c97704bac2b0
relation.isOrgUnitOfPublication.latestForDiscoveryca4c0298-cd82-48ee-a9c8-c97704bac2b0
unesp.author.lattes2640929291808415
unesp.author.orcid0000-0002-0592-542X[3]
unesp.author.orcid0000-0002-7629-0089[5]
unesp.author.orcid0000-0001-9413-5253[7]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquarapt
unesp.departmentFisiologia e Patologia - FOARpt

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