Scanning electron microscopy comparison of the resin–dentin interface using different specimen preparation methods
dc.contributor.author | Augusto, Marina G. [UNESP] | |
dc.contributor.author | Dantas, Debora C. B. [UNESP] | |
dc.contributor.author | de Andrade, Guilherme S. [UNESP] | |
dc.contributor.author | Matuda, Amanda G. N. [UNESP] | |
dc.contributor.author | Lopes, Stephanie R. [UNESP] | |
dc.contributor.author | Barcellos, Daphne C. [UNESP] | |
dc.contributor.author | Pucci, Cesar R. [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2020-12-12T01:21:59Z | |
dc.date.available | 2020-12-12T01:21:59Z | |
dc.date.issued | 2020-08-01 | |
dc.description.abstract | Microscopy has been widely used to complement the data of studies related to dentin bonding; however, different specimen preparation methods may influence the analysis. Aiming to contribute to the reported scenario, this study evaluated the effect of two specimen-sectioning methods (cleavage and diamond disk cut) on the quality of the scanning electron microscopy (SEM) images. Four crowns of human molars were selected and had an area of approximately 6 mm2 of dentin exposed. They were then divided into two groups according to the universal adhesive application: total and self-etching modes. Then, composite blocks were built up and the specimens were stored in deionized water to allow the postcuring. The specimens were further divided according to the sectioning method: cleavage or diamond disk cut. Four specimens were obtained from each tooth. They were desiccated, placed on aluminum stubs, sputter-coated with gold, and observed in a scanning electron microscope, with ×2000 of magnification. The quality of the SEM images were evaluated by two calibrated examiners and classified into four scores (1–4). Mann–Whitney test (p <.05) showed that the diamond disk provided significantly higher scores than cleavage, whereas no significant difference was observed when comparing the total-etching and self-etching modes of application. The diamond disk cut method is preferable to the cleavage method to ensure the quality of the SEM analysis in studies involving the resin–dentin interface. | en |
dc.description.affiliation | Department of Restorative Dentistry Institute of Science and Technology São Paulo State University-UNESP | |
dc.description.affiliation | Department of Prothodontics and Dental Materials Institute of Science and Technology São Paulo State University-UNESP | |
dc.description.affiliationUnesp | Department of Restorative Dentistry Institute of Science and Technology São Paulo State University-UNESP | |
dc.description.affiliationUnesp | Department of Prothodontics and Dental Materials Institute of Science and Technology São Paulo State University-UNESP | |
dc.format.extent | 945-948 | |
dc.identifier | http://dx.doi.org/10.1002/jemt.23488 | |
dc.identifier.citation | Microscopy Research and Technique, v. 83, n. 8, p. 945-948, 2020. | |
dc.identifier.doi | 10.1002/jemt.23488 | |
dc.identifier.issn | 1097-0029 | |
dc.identifier.issn | 1059-910X | |
dc.identifier.lattes | 1754020652874850 | |
dc.identifier.orcid | 0000-0003-4830-0400 | |
dc.identifier.scopus | 2-s2.0-85084139269 | |
dc.identifier.uri | http://hdl.handle.net/11449/198788 | |
dc.language.iso | eng | |
dc.relation.ispartof | Microscopy Research and Technique | |
dc.source | Scopus | |
dc.subject | adhesive system | |
dc.subject | bonding | |
dc.subject | dentin | |
dc.subject | scanning electron microscopy | |
dc.title | Scanning electron microscopy comparison of the resin–dentin interface using different specimen preparation methods | en |
dc.type | Artigo | |
dspace.entity.type | Publication | |
unesp.author.lattes | 1754020652874850[7] | |
unesp.author.orcid | 0000-0003-4830-0400[7] |