Xylooligosaccharide production from sugarcane bagasse and leaf using Aspergillus versicolor endoxylanase and diluted acid

Abstract

Xylooligosaccharides (XOS) are xylose oligomers that have numerous health benefits and can be produced by the acid or enzymatic hydrolysis of xylan-rich materials. This study aimed to extract xylan from sugarcane bagasse and leaf with 6% hydrogen peroxide in an alkaline medium and to hydrolyze it with Aspergillus versicolor endoxylanase and diluted acetic and sulfuric acids to produce XOS. The solubilization yields of xylan were 65.15% and 69.7% using sugarcane bagasse and leaf, respectively. The highest conversions of xylan from bagasse and leaf into XOS using acetic and sulfuric acids were 56.29% and 53.65%, and 40.16% and 45.37%, respectively. In enzymatic hydrolysis, the highest conversion yields were 67.43% and 69.71% for xylan from the bagasse and leaf, respectively. The results showed that acid and enzymatic hydrolysis resulted in high XOS yields; however, enzymatic hydrolysis of the degradation products did not occur.